Experiment Overview
| Repository ID: | FR-FCM-Z2CP | Experiment name: | Ma et al CD8+ T cell signalling panel experiment 2 | MIFlowCyt score: | 82.83% |
| Primary researcher: | Claire Ma | PI/manager: | Arianne Richard | Uploaded by: | Arianne Richard |
| Experiment dates: | 2019-02-05 - 2019-02-06 | Dataset uploaded: | Nov 2019 | Last updated: | Nov 2019 |
| Keywords: | [mass cytometry] [CyTOF] [CD8+ T cells] | Manuscripts: | [32412411] |
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| Organizations: |
Cambridge Institute for Medical Research, Department of Medicine, Cambridge, (UK)
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| Purpose: | We wished to understand how stimulation strength impacted the signalling pathways initiated downstream of TCR ligation. We stimulated OTI Rag1-deficient CD8+ T cells with 4 peptides (SIINFEKL (N4) = high potency, SIITFEKL (T4) = medium potency, SIIGFEKL (G4) = low potency, ASNENMDAM (NP68) = null) for 0, 1, 2, 4 or 6 hours and then examined their signalling using a custom mass cytometry panel. | ||||
| Conclusion: | We find that it is the rate of activation and not the organization of this programme that responds to signal strength. | ||||
| Comments: | None | ||||
| Funding: | This work was funded by the Wellcome Trust (grants [103930], [100140] and [217100] to G.M.G and grant 204017/Z/16/Z to C.Y.M); Cancer Research UK (core funding [A17197] to J.C.M); EMBL (core funding to J.C.M); the MRC (MR/P014178/1 to A.C.R); the ACT (grant [23/17 A (ii)] to C.Y.M). A.C.R also received a supplementary grant from the National Institute for Health Research [Cambridge Biomedical Research Centre at the Cambridge University Hospitals NHS Foundation Trust]. | ||||
| Quality control: | None | ||||
Experiment variables
| Timepoints | |
|---|---|
| · 0 hour | c01_SAMPLE_A_01_1_0.fcs · c01_SAMPLE_B_01_1_0.fcs · c02_SAMPLE_A_01_1_0.fcs · c02_SAMPLE_B_01_1_0.fcs |
| · 1 hour | c16_SAMPLE_A_01_1_0.fcs · c16_SAMPLE_B_01_1_0.fcs · c17_SAMPLE_A_01_1_0.fcs · c17_SAMPLE_B_01_1_0.fcs · c18_SAMPLE_A_01_1_0.fcs · c18_SAMPLE_B_01_1_0.fcs · c19_SAMPLE_A_01_1_0.fcs · c19_SAMPLE_B_01_1_0.fcs |
| · 2 hour | c03_SAMPLE_A_01_1_0.fcs · c03_SAMPLE_B_01_1_0.fcs · c04_SAMPLE_A_01_1_0.fcs · c04_SAMPLE_B_01_1_0.fcs · c05_SAMPLE_A_01_1_0.fcs · c05_SAMPLE_B_01_1_0.fcs · c06_SAMPLE_A_01_1_0.fcs · c06_SAMPLE_B_01_1_0.fcs · c07_SAMPLE_A_01_1_0.fcs · c07_SAMPLE_B_01_1_0.fcs |
| · 4 hour | c08_SAMPLE_A_01_1_0.fcs · c08_SAMPLE_B_01_1_0.fcs · c09_SAMPLE_A_01_1_0.fcs · c09_SAMPLE_B_01_1_0.fcs · c10_SAMPLE_A_01_1_0.fcs · c10_SAMPLE_B_01_1_0.fcs · c11_SAMPLE_A_01_1_0.fcs · c11_SAMPLE_B_01_1_0.fcs |
| · 6 hour | c12_SAMPLE_A_01_1_0.fcs · c12_SAMPLE_B_01_1_0.fcs · c13_SAMPLE_A_01_1_0.fcs · c13_SAMPLE_B_01_1_0.fcs · c14_SAMPLE_A_01_1_0.fcs · c14_SAMPLE_B_01_1_0.fcs · c15_SAMPLE_A_01_1_0.fcs · c15_SAMPLE_B_01_1_0.fcs |
| Conditions | |
|---|---|
| · N4 | c03_SAMPLE_A_01_1_0.fcs · c03_SAMPLE_B_01_1_0.fcs · c04_SAMPLE_A_01_1_0.fcs · c04_SAMPLE_B_01_1_0.fcs · c08_SAMPLE_A_01_1_0.fcs · c08_SAMPLE_B_01_1_0.fcs · c12_SAMPLE_A_01_1_0.fcs · c12_SAMPLE_B_01_1_0.fcs · c16_SAMPLE_A_01_1_0.fcs · c16_SAMPLE_B_01_1_0.fcs |
| · T4 | c05_SAMPLE_A_01_1_0.fcs · c05_SAMPLE_B_01_1_0.fcs · c09_SAMPLE_A_01_1_0.fcs · c09_SAMPLE_B_01_1_0.fcs · c13_SAMPLE_A_01_1_0.fcs · c13_SAMPLE_B_01_1_0.fcs · c17_SAMPLE_A_01_1_0.fcs · c17_SAMPLE_B_01_1_0.fcs |
| · G4 | c06_SAMPLE_A_01_1_0.fcs · c06_SAMPLE_B_01_1_0.fcs · c10_SAMPLE_A_01_1_0.fcs · c10_SAMPLE_B_01_1_0.fcs · c14_SAMPLE_A_01_1_0.fcs · c14_SAMPLE_B_01_1_0.fcs · c18_SAMPLE_A_01_1_0.fcs · c18_SAMPLE_B_01_1_0.fcs |
| · NP68 | c07_SAMPLE_A_01_1_0.fcs · c07_SAMPLE_B_01_1_0.fcs · c11_SAMPLE_A_01_1_0.fcs · c11_SAMPLE_B_01_1_0.fcs · c15_SAMPLE_A_01_1_0.fcs · c15_SAMPLE_B_01_1_0.fcs · c19_SAMPLE_A_01_1_0.fcs · c19_SAMPLE_B_01_1_0.fcs |
| · unstimulated | c01_SAMPLE_A_01_1_0.fcs · c01_SAMPLE_B_01_1_0.fcs · c02_SAMPLE_A_01_1_0.fcs · c02_SAMPLE_B_01_1_0.fcs |
