Experiment Overview
| Repository ID: | FR-FCM-Z2D6 | Experiment name: | Characterization of the impact of daclizumab beta on circulating natural killer cells by mass cytometry | MIFlowCyt score: | 53.46% |
| Primary researcher: | Thanmayi Ranganath | PI/manager: | Catherine Blish | Uploaded by: | Thanmayi Ranganath |
| Experiment dates: | 2015-12-01 - 2019-11-01 | Dataset uploaded: | Dec 2019 | Last updated: | Apr 2020 |
| Keywords: | [Clustering] [mass cytometry] [natural killer cells] [multiple sclerosis] [Big Data] [GLMM] | Manuscripts: | |||
| Organizations: |
Stanford University, Stanford, Stanford, CA (United States)
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| Purpose: | NK cells represent a heterogeneous population of lymphocytes with a range of phenotypes and functions. The goal of this study was to better understand how daclizumab beta altered the NK cell repertoire to provide further insight into the possible mechanism(s) of action in Multiple Sclerosis. | ||||
| Conclusion: | The data demonstrate that significant changes occur in NK cells in response to the increased IL-2 availability induced by daclizumab beta treatment. These data extend prior findings indicating that daclizumab beta treatment increases the frequency of the CD56bright population, which is not due to an increase of a specific subset of CD56bright NK cells. NK receptor expression is broadly altered across CD56bright NK cells, highlighting the unique phenotypic features of these expanded NK cells. The high expression of activation markers, activating receptors, and perforin could enhance the ability of these CD56bright NK cells to control RMS through cytolytic activity or other immunoregulatory functions. The deep profiling of NK cells performed here, including in the placebo group, can also serve as a reference for future studies of NK cell phenotype in the setting of RMS. | ||||
| Comments: | None | ||||
| Funding: | Biogen Idec | ||||
| Quality control: | All samples from pulled form 2 clinical trials | ||||
Experiment variables
| Conditions | |
|---|---|
| · HealthyDonor_Baseline | EV02.fcs · EV03.fcs · LS01.fcs · LS02.fcs · NZ01.fcs · NZ02.fcs · NZ03.fcs · NZ04.fcs · NZ05.fcs · NZ06.fcs · NZ07.fcs · NZ08.fcs · NZ09.fcs · NZ11.fcs · NZ13.fcs · NZ14.fcs |
| · Placebo_Baseline | 110007_B.fcs · 353004_B.fcs · 453026_B.fcs · 505030_B.fcs · 553003_B.fcs · 758014_B.fcs · 761005_B.fcs · 763006_B.fcs · 763007_B.fcs · 903004_B.fcs · 903008_B.fcs · 903010_B.fcs · 903012_B.fcs · 903015_B.fcs · 903020_B.fcs · 906012_B.fcs |
| · Placebo_24week | 110007_24.fcs · 353004_24.fcs · 453026_24.fcs · 505030_24.fcs · 553003_24.fcs · 761005_24.fcs · 763007_24.fcs · 903004_24.fcs · 903008_24.fcs · 903010_24.fcs · 903012_24.fcs · 903015_24.fcs · 903020_24.fcs · 906012_24.fcs |
| · Placebo_52week | 110007_52.fcs · 353004_52.fcs · 453026_52.fcs · 505030_52.fcs · 553003_52.fcs · 758014_52.fcs · 761005_52.fcs · 763006_52.fcs · 763007_52.fcs · 903004_52.fcs · 903008_52.fcs · 903010_52.fcs · 903012_52.fcs · 903015_52.fcs · 903020_52.fcs · 906012_52.fcs |
| · Drug_Baseline | 326004_B.fcs · 411003_B.fcs · 412007_B.fcs · 441008_B.fcs · 453004_B.fcs · 460006_B.fcs · 460008_B.fcs · 536016_B.fcs · 604032_B.fcs · 606014_B.fcs · 606023_B.fcs · 611038_B.fcs · 613009_B.fcs · 615006_B.fcs · 622003_B.fcs · 641007_B.fcs · 657007_B.fcs · 657012_B.fcs · 658005_B.fcs · 678008_B.fcs · 680004_B.fcs · 702001_B.fcs |
| · Drug_24week | 326004_24.fcs · 411003_24.fcs · 412007_24.fcs · 441008_24.fcs · 441009_24.fcs · 453004_24.fcs · 460006_24.fcs · 460008_24.fcs · 536016_24.fcs · 604032_24.fcs · 606014_24.fcs · 606023_24.fcs · 606025_24.fcs · 611038_24.fcs · 613009_24.fcs · 615006_24.fcs · 622003_24.fcs · 641007_24.fcs · 657007_24.fcs · 657012_24.fcs · 678007_24.fcs · 678008_24.fcs · 680004_24.fcs · 702001_24.fcs · 550003_24.fcs |
| · Drug_52week | 239001_48.fcs · 326004_48.fcs · 411003_48.fcs · 412007_48.fcs · 441008_48.fcs · 441009_48.fcs · 453004_48.fcs · 460006_52.fcs · 460008_52.fcs · 536016_48.fcs · 604032_48.fcs · 606014_48.fcs · 606023_48.fcs · 606025_48.fcs · 611038_48.fcs · 613009_48.fcs · 615006_48.fcs · 622003_48.fcs · 641007_48.fcs · 657007_48.fcs · 657012_48.fcs · 658005_48.fcs · 678007_48.fcs · 678008_48.fcs · 680004_48.fcs · 702001_48.fcs · 550003_48.fcs |
