Experiment Overview

Repository ID: FR-FCM-Z2FJ Experiment name: neonate immune phenotyping MIFlowCyt score: 21.00%
Primary researcher: Shraddha Kamdar PI/manager: Deena Gibbons Uploaded by: Shraddha Kamdar
Experiment dates: 2016-05-02 - 2019-09-30 Dataset uploaded: Feb 2020 Last updated: Feb 2020
Keywords: None Manuscripts:
Organizations: King's College London, King's College London, London, London (United Kingdom)
Purpose: To longitudinally phenotype the developing immune system in PBMCs from preterm babies
Conclusion: None
Comments: Sample nomenclature: Samples labelled Adult are Adult PBMCs Samples labelled B501302292 is labelled such that B denotes blood, 50 is a code and 13 denotes participant number. so B5013, B5022, B5023 etc are individual participants. Samples labelled BT/CE189 are PBMC samples from term babies. There are 7 flow panels. Tubes labelled 4B/5B denote BFA treated samples and 4P/5P denote PMA/ionomycin stimulated samples. Partially through the study, one of the fluorochromes on Panel 6 (tubes labelled 4B/4P) was changed from CD8 BV510 to CD161 BV510, however the name was not changed on the panel itself so still reads CD8 BV510. The samples where CD161 BV510 was used instead are B5021,B5022, B5028, B5041,B5047,B5054,B5055,B5069,B5025, B5026,B5029, B5038,B5058,B5072,B5011,B5023,B5086,B5092, B5112, B5042, B5039,B5040, B5043,B5044,B5051,B5059,B5060,B5066,B5070,B5076,B5049.B5101, Adults BB,IZ,RB,ALG and all term samples.
Funding: Not disclosed
Quality control: None

Experiment variables

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