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Comments:
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Treat HeLa and Jurkat cell lines with SOM3B reagents. Each cell line was labeled with 5 uM IdU, 2 mM BRU, and 1 ug/mL puromycin, for 30 minutes, followed immediately with cell collection and fixation. Biosynthesis activity was quantified by measuring incorporation of IdU (DNA synthesis) by directly measuring elemental Iodine (I-127) by mass cytometry. Antibodies detecting incorporated BRU and puromycin, each conjugated to a specific purified isotope was used to quantify RNA and protein synthesis, respectively. Detailed methods describing SOM3B labeling can be found in the linked paper.
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