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Experiment Overview

Repository ID: FR-FCM-Z2HS Experiment name: PSG effect on Th17-polarized CD4 cells MIFlowCyt score: 36.00%
Primary researcher: Valeria Timganova PI/manager: Valeria Timganova Uploaded by: Valeria Timganova
Experiment dates: 2018-03-19 - 2018-04-02 Dataset uploaded: Mar 2020 Last updated: Mar 2020
Keywords: [Th17] [pregnancy-specific β1-glycoprotein] [RORgt] [Ki67] Manuscripts:
Organizations: IEGM UrBr RAS, IEGM UrBr RAS, Perm, Perm (Russia)
Purpose: to study the effects of PSGs on the Th17 polarization of CD4+ cells
Conclusion: Native human pregnancy-specific β1-glycoprotein preparation (PSG) at a concentration of 100 µg/mL was shown to decrease the frequency of Th17 (RORγτ+) in CD4+ cell culture and to suppress the proliferation of these cells (RORγτ+Ki-67+), along with proliferation of other cells (Ki-67+) (n=11). A PSG concentration of 10 µg/mL showed a similar effect, decreasing the frequency of Ki-67+ and RORγτ+Ki67+ cells.
Comments: None
Funding: Not disclosed
Quality control: CytoFLEX Daily QC Fluorospheres


Experiment variables

Doses
· 100 µg/ml PSG100_1 donor.fcs · PSG100_10 donor.fcs · PSG100_11 donor.fcs · PSG100_2 donor.fcs · PSG100_3 donor.fcs · PSG100_4 donor.fcs · PSG100_5 donor.fcs · PSG100_6 donor.fcs · PSG100_7 donor.fcs · PSG100_8 donor.fcs · PSG100_9 donor.fcs
· 10 µg/ml PSG10_1 donor.fcs · PSG10_10 donor.fcs · PSG10_11 donor.fcs · PSG10_2 donor.fcs · PSG10_3 donor1.fcs · PSG10_4 donor.fcs · PSG10_5 donor.fcs · PSG10_6 donor.fcs · PSG10_7 donor.fcs · PSG10_8 donor.fcs · PSG10_9 donor.fcs
· 1 µg/ml PSG1_1 donor.fcs · PSG1_10 donor.fcs · PSG1_11 donor.fcs · PSG1_2 donor.fcs · PSG1_3 donor.fcs · PSG1_4 donor.fcs · PSG1_5 donor.fcs · PSG1_6 donor.fcs · PSG1_7 donor.fcs · PSG1_8 donor.fcs · PSG1_9 donor.fcs
· 0 µg/ml K akt stained both 10 donor.fcs · K akt stained both 11 donor.fcs · K akt stained both 4 donor.fcs · K akt stained both 5 donor.fcs · K akt stained both 6 donor.fcs · K akt stained both 7 donor.fcs · K akt stained both 8 donor.fcs · K akt stained both 9 donor.fcs · K_akt_stained both_1 donor.fcs · K_akt_stained both_2 donor.fcs · K_akt_stained both_3 donor.fcs

Individuals
· 1 K_akt_not stained_1 donor.fcs · K_akt_stained Ki67_1 donor.fcs · K_akt_stained RORgt_1 donor.fcs · K_akt_stained both_1 donor.fcs · K_na_not stained_1 donor.fcs · K_na_stained_1 donor.fcs · PSG100_1 donor.fcs · PSG10_1 donor.fcs · PSG1_1 donor.fcs
· 2 K_akt_not stained_2 donor.fcs · K_akt_stained Ki 67_2 donor.fcs · K_akt_stained both_2 donor.fcs · K_akt_stainedRORgt_2 donor.fcs · K_na_not stained_2 donor.fcs · K_na_stained_2 donor.fcs · PSG100_2 donor.fcs · PSG10_2 donor.fcs · PSG1_2 donor.fcs
· 3 K_akt_not stained_3 donor.fcs · K_akt_stained Ki67_3 donor.fcs · K_akt_stained RORgt_3 donor.fcs · K_akt_stained both_3 donor.fcs · K_na_ stained_3 donor.fcs · K_na_not stained_3 donor.fcs · PSG100_3 donor.fcs · PSG10_3 donor1.fcs · PSG1_3 donor.fcs
· 4 K akt not stained 4 donor.fcs · K akt stained both 4 donor.fcs · K na not stained 4 donor.fcs · K na stained 4 donor.fcs · PSG100_4 donor.fcs · PSG10_4 donor.fcs · PSG1_4 donor.fcs
· 5 K akt not stained 5 donor.fcs · K akt stained both 5 donor.fcs · K na not stained 5 donor.fcs · K na stained 5 donor.fcs · PSG100_5 donor.fcs · PSG10_5 donor.fcs · PSG1_5 donor.fcs
· 6 K akt not stained 6 donor.fcs · K akt stained both 6 donor.fcs · K na not stained 6 donor.fcs · K na stained 6 donor.fcs · PSG100_6 donor.fcs · PSG10_6 donor.fcs · PSG1_6 donor.fcs
· 7 K akt not stained 7 donor.fcs · K akt stained both 7 donor.fcs · K na not stained 7 donor.fcs · K na stained 7 donor.fcs · PSG100_7 donor.fcs · PSG10_7 donor.fcs · PSG1_7 donor.fcs
· 8 K akt not stained 8 donor.fcs · K akt stained both 8 donor.fcs · K na not stained 8 donor.fcs · K na stained 8 donor.fcs · PSG100_8 donor.fcs · PSG10_8 donor.fcs · PSG1_8 donor.fcs
· 9 K akt not stained 9 donor.fcs · K akt stained both 9 donor.fcs · K na not stained 9 donor.fcs · K na stained 9 donor.fcs · PSG100_9 donor.fcs · PSG10_9 donor.fcs · PSG1_9 donor.fcs
· 10 K akt not stained 10 donor.fcs · K akt stained both 10 donor.fcs · K na not stained 10 donor.fcs · K na stained 10 donor.fcs · PSG100_10 donor.fcs · PSG10_10 donor.fcs · PSG1_10 donor.fcs
· 11 K akt not stained 11 donor.fcs · K akt stained both 11 donor.fcs · K na not stained 11 donor.fcs · K na stained 11 donor.fcs · PSG100_11 donor.fcs · PSG10_11 donor.fcs · PSG1_11 donor.fcs

Conditions
· not activated cells K na not stained 10 donor.fcs · K na not stained 11 donor.fcs · K na not stained 4 donor.fcs · K na not stained 5 donor.fcs · K na not stained 6 donor.fcs · K na not stained 7 donor.fcs · K na not stained 8 donor.fcs · K na not stained 9 donor.fcs · K na stained 10 donor.fcs · K na stained 11 donor.fcs · K na stained 4 donor.fcs · K na stained 5 donor.fcs · K na stained 6 donor.fcs · K na stained 8 donor.fcs · K na stained 9 donor.fcs · K_na_ stained_3 donor.fcs · K_na_not stained_1 donor.fcs · K_na_not stained_2 donor.fcs · K_na_not stained_3 donor.fcs · K_na_stained_1 donor.fcs · K_na_stained_2 donor.fcs

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