Experiment Overview
| Repository ID: | FR-FCM-Z2HS | Experiment name: | PSG effect on Th17-polarized CD4 cells | MIFlowCyt score: | 36.00% |
| Primary researcher: | Valeria Timganova | PI/manager: | Valeria Timganova | Uploaded by: | Valeria Timganova |
| Experiment dates: | 2018-03-19 - 2018-04-02 | Dataset uploaded: | Mar 2020 | Last updated: | Mar 2020 |
| Keywords: | [Th17] [pregnancy-specific β1-glycoprotein] [RORgt] [Ki67] | Manuscripts: | |||
| Organizations: |
IEGM UrBr RAS, IEGM UrBr RAS, Perm, Perm (Russia)
|
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| Purpose: | to study the effects of PSGs on the Th17 polarization of CD4+ cells | ||||
| Conclusion: | Native human pregnancy-specific β1-glycoprotein preparation (PSG) at a concentration of 100 µg/mL was shown to decrease the frequency of Th17 (RORγτ+) in CD4+ cell culture and to suppress the proliferation of these cells (RORγτ+Ki-67+), along with proliferation of other cells (Ki-67+) (n=11). A PSG concentration of 10 µg/mL showed a similar effect, decreasing the frequency of Ki-67+ and RORγτ+Ki67+ cells. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | CytoFLEX Daily QC Fluorospheres | ||||
Experiment variables
| Doses | |
|---|---|
| · 100 µg/ml | PSG100_1 donor.fcs · PSG100_10 donor.fcs · PSG100_11 donor.fcs · PSG100_2 donor.fcs · PSG100_3 donor.fcs · PSG100_4 donor.fcs · PSG100_5 donor.fcs · PSG100_6 donor.fcs · PSG100_7 donor.fcs · PSG100_8 donor.fcs · PSG100_9 donor.fcs |
| · 10 µg/ml | PSG10_1 donor.fcs · PSG10_10 donor.fcs · PSG10_11 donor.fcs · PSG10_2 donor.fcs · PSG10_3 donor1.fcs · PSG10_4 donor.fcs · PSG10_5 donor.fcs · PSG10_6 donor.fcs · PSG10_7 donor.fcs · PSG10_8 donor.fcs · PSG10_9 donor.fcs |
| · 1 µg/ml | PSG1_1 donor.fcs · PSG1_10 donor.fcs · PSG1_11 donor.fcs · PSG1_2 donor.fcs · PSG1_3 donor.fcs · PSG1_4 donor.fcs · PSG1_5 donor.fcs · PSG1_6 donor.fcs · PSG1_7 donor.fcs · PSG1_8 donor.fcs · PSG1_9 donor.fcs |
| · 0 µg/ml | K akt stained both 10 donor.fcs · K akt stained both 11 donor.fcs · K akt stained both 4 donor.fcs · K akt stained both 5 donor.fcs · K akt stained both 6 donor.fcs · K akt stained both 7 donor.fcs · K akt stained both 8 donor.fcs · K akt stained both 9 donor.fcs · K_akt_stained both_1 donor.fcs · K_akt_stained both_2 donor.fcs · K_akt_stained both_3 donor.fcs |
| Individuals | |
|---|---|
| · 1 | K_akt_not stained_1 donor.fcs · K_akt_stained Ki67_1 donor.fcs · K_akt_stained RORgt_1 donor.fcs · K_akt_stained both_1 donor.fcs · K_na_not stained_1 donor.fcs · K_na_stained_1 donor.fcs · PSG100_1 donor.fcs · PSG10_1 donor.fcs · PSG1_1 donor.fcs |
| · 2 | K_akt_not stained_2 donor.fcs · K_akt_stained Ki 67_2 donor.fcs · K_akt_stained both_2 donor.fcs · K_akt_stainedRORgt_2 donor.fcs · K_na_not stained_2 donor.fcs · K_na_stained_2 donor.fcs · PSG100_2 donor.fcs · PSG10_2 donor.fcs · PSG1_2 donor.fcs |
| · 3 | K_akt_not stained_3 donor.fcs · K_akt_stained Ki67_3 donor.fcs · K_akt_stained RORgt_3 donor.fcs · K_akt_stained both_3 donor.fcs · K_na_ stained_3 donor.fcs · K_na_not stained_3 donor.fcs · PSG100_3 donor.fcs · PSG10_3 donor1.fcs · PSG1_3 donor.fcs |
| · 4 | K akt not stained 4 donor.fcs · K akt stained both 4 donor.fcs · K na not stained 4 donor.fcs · K na stained 4 donor.fcs · PSG100_4 donor.fcs · PSG10_4 donor.fcs · PSG1_4 donor.fcs |
| · 5 | K akt not stained 5 donor.fcs · K akt stained both 5 donor.fcs · K na not stained 5 donor.fcs · K na stained 5 donor.fcs · PSG100_5 donor.fcs · PSG10_5 donor.fcs · PSG1_5 donor.fcs |
| · 6 | K akt not stained 6 donor.fcs · K akt stained both 6 donor.fcs · K na not stained 6 donor.fcs · K na stained 6 donor.fcs · PSG100_6 donor.fcs · PSG10_6 donor.fcs · PSG1_6 donor.fcs |
| · 7 | K akt not stained 7 donor.fcs · K akt stained both 7 donor.fcs · K na not stained 7 donor.fcs · K na stained 7 donor.fcs · PSG100_7 donor.fcs · PSG10_7 donor.fcs · PSG1_7 donor.fcs |
| · 8 | K akt not stained 8 donor.fcs · K akt stained both 8 donor.fcs · K na not stained 8 donor.fcs · K na stained 8 donor.fcs · PSG100_8 donor.fcs · PSG10_8 donor.fcs · PSG1_8 donor.fcs |
| · 9 | K akt not stained 9 donor.fcs · K akt stained both 9 donor.fcs · K na not stained 9 donor.fcs · K na stained 9 donor.fcs · PSG100_9 donor.fcs · PSG10_9 donor.fcs · PSG1_9 donor.fcs |
| · 10 | K akt not stained 10 donor.fcs · K akt stained both 10 donor.fcs · K na not stained 10 donor.fcs · K na stained 10 donor.fcs · PSG100_10 donor.fcs · PSG10_10 donor.fcs · PSG1_10 donor.fcs |
| · 11 | K akt not stained 11 donor.fcs · K akt stained both 11 donor.fcs · K na not stained 11 donor.fcs · K na stained 11 donor.fcs · PSG100_11 donor.fcs · PSG10_11 donor.fcs · PSG1_11 donor.fcs |
| Conditions | |
|---|---|
| · not activated cells | K na not stained 10 donor.fcs · K na not stained 11 donor.fcs · K na not stained 4 donor.fcs · K na not stained 5 donor.fcs · K na not stained 6 donor.fcs · K na not stained 7 donor.fcs · K na not stained 8 donor.fcs · K na not stained 9 donor.fcs · K na stained 10 donor.fcs · K na stained 11 donor.fcs · K na stained 4 donor.fcs · K na stained 5 donor.fcs · K na stained 6 donor.fcs · K na stained 8 donor.fcs · K na stained 9 donor.fcs · K_na_ stained_3 donor.fcs · K_na_not stained_1 donor.fcs · K_na_not stained_2 donor.fcs · K_na_not stained_3 donor.fcs · K_na_stained_1 donor.fcs · K_na_stained_2 donor.fcs |
