Experiment Overview
| Repository ID: | FR-FCM-Z2JN | Experiment name: | Major populations of equine PBMC | MIFlowCyt score: | 93.54% |
| Primary researcher: | Joy Tomlinson | PI/manager: | Brad Rosenberg | Uploaded by: | Joy Tomlinson |
| Experiment dates: | 2017-08-21 - 2018-11-13 | Dataset uploaded: | Apr 2020 | Last updated: | Apr 2024 |
| Keywords: | [B cells] [T cell] [PBMC] [NK cells] [T cells] [Tbet] | Manuscripts: | |||
| Organizations: |
Cornell University College of Veterinary Medicine, Baker Institute for Animal Health, Ithaca, NY (United States)
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| Purpose: | Phenotype equine PBMC by flow cytometry for comparison to single cell RNA-Sequencing. Panel M was used to determine the proportion of T cells, B cells, monocytes, NK-like cells (CD3+CD16+), and alternatively activated monocytes (CD14+CD16+). Panel T was used to determine CD4+ and CD8+ T cells and replicating Ki67+ T cells. Panel B1 and Panel B2 were used to characterize Tbet expression in B cells and co-expression of T bet with other markers. | ||||
| Conclusion: | Flow cytometry and scRNA-Seq quantification of these cell populations showed very high correlation. Horses have a markedly high proportion of Tbet+ B cells. | ||||
| Comments: | None | ||||
| Funding: | This study was supported by grants from the Agriculture and Food Research Initiative Competitive Grant no. 2016-67015-24765 from the USDA National Institute of Food and Agriculture and the Jack Lowe Equine Health Funds/Mollie Wilmot Equine Research Fund. J.E.T. was supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under Award number K08AI141767. The content is solely the responsibility of the authors and does not necessarily represent the official views of the funders, including the National Institutes of Health. | ||||
| Quality control: | Seven biological replicates with different ages, sexes, and breeds were used. Single color compensation controls were included. | ||||
Experiment variables
| Individuals | |
|---|---|
| · Horse 1 | Horse 1 Panel M.fcs · Horse 1 Panel B1.fcs · Horse 1 Panel B2.fcs · Horse 1 Panel T.fcs |
| · Horse 2 | Horse 2 Panel M.fcs · Horse 2 Panel B1.fcs · Horse 2 Panel B2.fcs · Horse 2 Panel T.fcs |
| · Horse 3 | Horse 3 Panel M.fcs · Horse 3 Panel B1.fcs · Horse 3 Panel B2.fcs · Horse 3 Panel T.fcs |
| · Horse 4 | Horse 4 Panel M.fcs · Horse 4 Panel B1.fcs · Horse 4 Panel B2.fcs · Horse 4 Panel T.fcs |
| · Horse 5 | Horse 5 Panel M.fcs · Horse 5 Panel B1.fcs · Horse 5 Panel B2.fcs · Horse 5 Panel T.fcs |
| · Horse 6 | Horse 6 Panel M.fcs · Horse 6 Panel B1.fcs · Horse 6 Panel B2.fcs · Horse 6 Panel T.fcs |
| · Horse 7 | Horse 7 Panel M.fcs · Horse 7 Panel B1.fcs · Horse 7 Panel B2.fcs · Horse 7 Panel T.fcs |
| · controls | FMO Panel M-CD16.fcs · FMO Panel T-Ki67.fcs |
