Experiment Overview
| Repository ID: | FR-FCM-Z2LU | Experiment name: | Figure 3a | MIFlowCyt score: | 31.73% |
| Primary researcher: | Román Ramos Báez | PI/manager: | Britney Moss | Uploaded by: | Román Ramos Báez |
| Experiment dates: | 2019-09-27 - 2019-09-27 | Dataset uploaded: | May 2020 | Last updated: | Jun 2020 |
| Keywords: | [flow cytometry] [fluorescence] [Saccharomyces cerevisiae] [auxin] [TIR1/AFBs] | Manuscripts: | [32123041] [PMC7140906] | ||
| Organizations: |
Whitman College, Department of Biology , Walla Walla, WA (United States)
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| Purpose: | In this experiment, we test the differences in the degradation rates between different TIR1/AFB receptors from different species. | ||||
| Conclusion: | The maize auxin receptor ZmAFB2/3 b1 exhibited a higher basal activity compared with the Arabidopsis ortholog. A, ZmAFB2/3 b1 was sensitive to auxin and exhibited faster degradation than AtAFB2. ZmAFB2/3 a was not sensitive to auxin. Yeast coexpressing an Arabidopsis or maize auxin receptor and YFP-ZmIAA10 were treated with 1 μm auxin or mock (0), and fluorescence was measured on a flow cytometer. | ||||
| Comments: | r1_.... means rep1 ...1_.... means plate 1 | ||||
| Funding: | National Science Foundation IOS-1546873; IOS-1456950 M.J. Murdock Charitable Trust Howard Hughes Medical Institute GT11355 | ||||
| Quality control: | Yeast autofluorescence was cancelled out using non-fluorescent yeast. All yeast strains were tested for basal fluorescence prior to treatment from over 50 minutes of growth. | ||||
