Experiment Overview
| Repository ID: | FR-FCM-Z2M5 | Experiment name: | Figure 1d | MIFlowCyt score: | 31.54% |
| Primary researcher: | Román Ramos Báez | PI/manager: | Britney Moss | Uploaded by: | Román Ramos Báez |
| Experiment dates: | 2017-09-12 - 2019-11-04 | Dataset uploaded: | May 2020 | Last updated: | Jun 2020 |
| Keywords: | [flow cytometry] [fluorescence] [Synthetic Biology] [auxin] [Aux/IAAs] | Manuscripts: | [32123041] [PMC7140906] | ||
| Organizations: |
Whitman College, Department of Biology , Walla Walla, WA (United States)
|
||||
| Purpose: | Auxin repressors expressed in maize inflorescence exhibited variable auxin-induced degradation dynamics. D, The 16 ZmIAAs coexpressed with Arabidopsis TIR1 were degraded in response to auxin. Fluorescence measurements were obtained 2 h post-auxin exposure on a flow cytometer. Data from two replicates are shown; error bars represent se. AU, Absorbance units. | ||||
| Conclusion: | D, The 16 ZmIAAs coexpressed with Arabidopsis TIR1 were degraded in response to auxin. Fluorescence measurements were obtained 2 h post-auxin exposure on a flow cytometer. Data from two replicates are shown; error bars represent se. AU, Absorbance units. | ||||
| Comments: | None | ||||
| Funding: | National Science Foundation IOS-1546873; IOS-1456950 M.J. Murdock Charitable Trust Howard Hughes Medical Institute GT11355 | ||||
| Quality control: | Yeast autofluorescence was cancelled out using non-fluorescent yeast. All yeast strains were tested for basal fluorescence prior to treatment from over 50 minutes of growth. | ||||
