Experiment Overview
| Repository ID: | FR-FCM-Z2T8 | Experiment name: | Mixed Dark Fermentation (DF) culture for biohydrogen production: morphology and physiological state assessment (DiBAC4(3)). | MIFlowCyt score: | 33.00% |
| Primary researcher: | Carlos Enrique Gomez Camacho | PI/manager: | Carlos Enrique Gomez Camacho | Uploaded by: | Carlos Enrique Gomez Camacho |
| Experiment dates: | 2018-05-22 - 2018-05-29 | Dataset uploaded: | Aug 2020 | Last updated: | Aug 2020 |
| Keywords: | [dark fermentation] [biohydrogen] [physiological state] [DiBAC4(3)] | Manuscripts: | |||
| Organizations: |
Politecnico di Torino / Technische Universität Berlin , Dep. Applied Science and Technology / Chair of Bioprocess Engineering, Torino / Berlin, TO / BE (ITALY / GERMANY)
|
||||
| Purpose: | Monitoring the physiological state in the Dark Fermentation of maize/grass silage using flow cytometry under ph-regulated (system A) and non-regulated conditions (system B). The study comprehends morphological observations (sporulation) and the quantification of depolarized cells using DiBAC4(3) at different time-points. | ||||
| Conclusion: | Morphological observations can be useful to gain insights and improve cellular performance in mixed culture systems by identifiying sporulation stages. The use of DiBAC4(3) staining seems to be an useful indicator for cmixed microbial cultures and can potentially facilitate monitoring of their PS during complex fermentations. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Timepoints | |
|---|---|
| · I | A_Timepoint I_BOX_rep1.fcs · A_Timepoint I_BOX_rep2.fcs · A_Timepoint I_BOX_rep3.fcs · A_Timepoint I_rep1.fcs · A_Timepoint I_rep2.fcs · A_Timepoint I_rep3.fcs · B_Timepoint I_BOX_rep1.fcs · B_Timepoint I_BOX_rep2.fcs · B_Timepoint I_BOX_rep3.fcs · B_Timepoint I_rep1.fcs · B_Timepoint I_rep2.fcs · B_Timepoint I_rep3.fcs |
| · II | A_Timepoint II_BOX_rep1.fcs · A_Timepoint II_BOX_rep2.fcs · A_Timepoint II_BOX_rep3.fcs · A_Timepoint II_rep1.fcs · A_Timepoint II_rep2.fcs · A_Timepoint II_rep3.fcs · B_Timepoint II_BOX_rep1.fcs · B_Timepoint II_BOX_rep2.fcs · B_Timepoint II_BOX_rep3.fcs · B_Timepoint II_rep1.fcs · B_Timepoint II_rep2.fcs · B_Timepoint II_rep3.fcs |
| · III | A_Timepoint III_BOX_rep1.fcs · A_Timepoint III_BOX_rep2.fcs · A_Timepoint III_BOX_rep3.fcs · A_Timepoint III_rep1.fcs · A_Timepoint III_rep2.fcs · A_Timepoint III_rep3.fcs · B_Timepoint III_BOX_rep1.fcs · B_Timepoint III_BOX_rep2.fcs · B_Timepoint III_BOX_rep3.fcs · B_Timepoint III_rep1.fcs · B_Timepoint III_rep2.fcs · B_Timepoint III_rep3.fcs |
| · 0 | Acid-treated AD Sludge (spore-enriched)_rep1.fcs · Acid-treated AD Sludge (spore-enriched)_rep2.fcs · Acid-treated AD Sludge (spore-enriched)_rep3.fcs |
| Individuals | |
|---|---|
| · A | A_Timepoint III_BOX_rep1.fcs · A_Timepoint III_BOX_rep2.fcs · A_Timepoint III_BOX_rep3.fcs · A_Timepoint III_rep1.fcs · A_Timepoint III_rep2.fcs · A_Timepoint III_rep3.fcs · A_Timepoint II_BOX_rep1.fcs · A_Timepoint II_BOX_rep2.fcs · A_Timepoint II_BOX_rep3.fcs · A_Timepoint II_rep1.fcs · A_Timepoint II_rep2.fcs · A_Timepoint II_rep3.fcs · A_Timepoint I_BOX_rep1.fcs · A_Timepoint I_BOX_rep2.fcs · A_Timepoint I_BOX_rep3.fcs · A_Timepoint I_rep1.fcs · A_Timepoint I_rep2.fcs · A_Timepoint I_rep3.fcs |
| · B | B_Timepoint III_BOX_rep1.fcs · B_Timepoint III_BOX_rep2.fcs · B_Timepoint III_BOX_rep3.fcs · B_Timepoint III_rep1.fcs · B_Timepoint III_rep2.fcs · B_Timepoint III_rep3.fcs · B_Timepoint II_BOX_rep1.fcs · B_Timepoint II_BOX_rep2.fcs · B_Timepoint II_BOX_rep3.fcs · B_Timepoint II_rep1.fcs · B_Timepoint II_rep2.fcs · B_Timepoint II_rep3.fcs · B_Timepoint I_BOX_rep1.fcs · B_Timepoint I_BOX_rep2.fcs · B_Timepoint I_BOX_rep3.fcs · B_Timepoint I_rep1.fcs · B_Timepoint I_rep2.fcs · B_Timepoint I_rep3.fcs |
| · 0 | Acid-treated AD Sludge (spore-enriched)_rep1.fcs · Acid-treated AD Sludge (spore-enriched)_rep2.fcs · Acid-treated AD Sludge (spore-enriched)_rep3.fcs |
