Experiment Overview
| Repository ID: | FR-FCM-Z2TW | Experiment name: | The impact of RBS on biosensor dynamic range | MIFlowCyt score: | 99.00% |
| Primary researcher: | nn ding | PI/manager: | Shenghu Zhou | Uploaded by: | nn ding |
| Experiment dates: | 2019-04-01 - 2020-08-15 | Dataset uploaded: | Aug 2020 | Last updated: | Aug 2020 |
| Keywords: | [biosensor] [dynamic range] [RBS] [translation] | Manuscripts: | |||
| Organizations: |
jiangnan university, National Engineering Laboratory for Cereal Fermentation Technology (NELCF), wuxi, Jiangshu (china)
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| Purpose: | In order to whether RBS is an important factor affecting biosenosr dynamic range. | ||||
| Conclusion: | RBS is an important factor affecting biosenosr dynamic range, and RBS is also an important factor affecting translation and folding of protein. | ||||
| Comments: | biosensor dynamic range | ||||
| Funding: | the National Key R&D Program of China (2019YFA0905502), the National Natural Science Foundation of China (21877053, 31900066), National First-class Discipline Program of Light Industry Technology and Engineering (LITE2018-24), Top-Notch Academic Programs Project of Jiangsu Higher Education Institutions (TAPP), Fundamental Research Funds for the Central Universities (JUSRP51705A, JUSRP12056), and Open Project Program of China-Canada Joint Lab of Food Nutrition and Health, Beijing Technology and Business University (BTBU) and Jiangsu Province Science Foundation for Youths (BK20150159) | ||||
| Quality control: | FCS collection for software testing | Remove background and adhesion | |||
Experiment variables
| Conditions | |
|---|---|
| · GroELS | 200805_RG10+GroELS(1)_001.fcs · 200805_RG10-GroELS(5)_006.fcs · 200805_RM10+GroELS(2)_039.fcs · 200805_RM10-GroELS(4)_037.fcs · 200805_RM2+GroELS(3)_016.fcs · 200805_RM2-GroELS(1)_010.fcs · 200805_RR3+GroELS(2)_023.fcs · 200805_RR3-GroELS(2)_019.fcs · Specimen_001_RR8+GroELS(3)_011.fcs · Specimen_001_RR8-GroELS(3)_010.fcs |
| Individuals | |
|---|---|
| · GA | Specimen_001_GA sample-1_002.fcs |
| Doses | |
|---|---|
| · I | Specimen_001_BJ04M10+arabinose_048.fcs · Specimen_001_BJ04M10+glucarate_002.fcs · Specimen_001_BJ04M10+glycolate_086.fcs · Specimen_001_BJ04M10-arabinose_047.fcs · Specimen_001_BJ04M10-glucarate_001.fcs · Specimen_001_BJ04M10-glycolate_085.fcs · Specimen_001_BJ04Rg+arabinose_072.fcs · Specimen_001_BJ04Rg+glucarate_026.fcs · Specimen_001_BJ04Rg+glycolate_106.fcs · Specimen_001_BJ04Rg-arabinose_071.fcs · Specimen_001_BJ04Rg-glucarate_025.fcs · Specimen_001_BJ04Rg-glycolate_105.fcs · Specimen_001_G10M10+arabinose_050.fcs · Specimen_001_G10M10+glucarate_028.fcs · Specimen_001_G10M10+glycolate_088.fcs · Specimen_001_G10M10-arabinose_049.fcs · Specimen_001_G10M10-glucarate_027.fcs · Specimen_001_G10M10-glycolate_087.fcs · Specimen_001_G10Rc+arabinose_052.fcs · Specimen_001_G10Rc+glucarate_030.fcs · Specimen_001_G10Rc+glycolate_090.fcs · Specimen_001_G10Rc-arabinose_051.fcs · Specimen_001_G10Rc-glucarate_029.fcs · Specimen_001_G10Rc-glycolate_089.fcs · Specimen_001_G10Rg+arabinose_054.fcs · Specimen_001_G10Rg+glucarate_008.fcs · Specimen_001_G10Rg+glycolate_092.fcs · Specimen_001_G10Rg-arabinose_053.fcs · Specimen_001_G10Rg-glucarate_007.fcs · Specimen_001_G10Rg-glycolate_040.fcs · Specimen_001_G10RpR+arabinose_056.fcs · Specimen_001_G10RpR+glucarate_010.fcs · Specimen_001_G10RpR+glycolate_094.fcs · Specimen_001_G10RpR-arabinose_055.fcs · Specimen_001_G10RpR-glucarate_009.fcs · Specimen_001_G10RpR-glycolate_093.fcs · Specimen_001_G10RpT+arabinose_082.fcs · Specimen_001_G10RpT+glucarate(1)_001.fcs · Specimen_001_G10RpT+glycolate_108.fcs · Specimen_001_G10RpT-arabinose_081.fcs · Specimen_001_G10RpT-glucarate(1)_001.fcs · Specimen_001_G10RpT-glycolate_107.fcs · Specimen_001_GA sample-1_002.fcs · Specimen_001_I-glucarate_001.fcs · Specimen_001_II-glucarate_002.fcs · Specimen_001_III-glucarate_003.fcs · Specimen_001_IV-glucarate_004.fcs · Specimen_001_M10G10+arabinose_058.fcs · Specimen_001_M10G10+glucarate_016.fcs · Specimen_001_M10G10+glycolate_096.fcs · Specimen_001_M10G10-arabinose_057.fcs · Specimen_001_M10G10-glucarate_015.fcs · Specimen_001_M10G10-glycolate_095.fcs · Specimen_001_M10Rg+arabinose_060.fcs · Specimen_001_M10Rg+glucarate_014.fcs · Specimen_001_M10Rg+glycolate_098.fcs · Specimen_001_M10Rg-arabinose_059.fcs · Specimen_001_M10Rg-glucarate_013.fcs · Specimen_001_M10Rg-glycolate_068.fcs · Specimen_001_M10RpR+arabinose_007.fcs · Specimen_001_M10RpR+glucarate_006.fcs · Specimen_001_M10RpR+glycolate_035.fcs · Specimen_001_M10RpR-arabinose_008.fcs · Specimen_001_M10RpR-glucarate_005.fcs · Specimen_001_M10RpR-glycolate_034.fcs · Specimen_001_RR8+GroELS(3)_011.fcs · Specimen_001_RR8-GroELS(3)_010.fcs · Specimen_001_RcG10+arabinose_018.fcs · Specimen_001_RcG10+glucarate(1)_001.fcs · Specimen_001_RcG10+glycolate_100.fcs · Specimen_001_RcG10-arabinose_017.fcs · Specimen_001_RcG10-glucarate(1)_001.fcs · Specimen_001_RcG10-glycolate_099.fcs · Specimen_001_RcRc+arabinose_011.fcs · Specimen_001_RcRc+glucarate_020.fcs · Specimen_001_RcRc+glycolate_102.fcs · Specimen_001_RcRc-arabinose_009.fcs · Specimen_001_RcRc-glucarate_019.fcs · Specimen_001_RcRc-glycolate_101.fcs · Specimen_001_RcRg+arabinose_068.fcs · Specimen_001_RcRg+glucarate_022.fcs · Specimen_001_RcRg+glycolate_057.fcs · Specimen_001_RcRg-arabinose_067.fcs · Specimen_001_RcRg-glucarate_021.fcs · Specimen_001_RcRg-glycolate_056.fcs · Specimen_001_RgBJ08+arabinose_074.fcs · Specimen_001_RgBJ08+glucarate_028.fcs · Specimen_001_RgBJ08+glycolate_110.fcs · Specimen_001_RgBJ08-arabinose_073.fcs · Specimen_001_RgBJ08-glucarate_027.fcs · Specimen_001_RgBJ08-glycolate_109.fcs · Specimen_001_RgG10+arabinose_076.fcs · Specimen_001_RgG10+glucarate(1)_001.fcs · Specimen_001_RgG10+glycolate_001.fcs · Specimen_001_RgG10-arabinose_075.fcs · Specimen_001_RgG10-glucarate(1)_001.fcs · Specimen_001_RgG10-glycolate_001.fcs · Specimen_001_RgRpR+arabinose_016.fcs · Specimen_001_RgRpR+glucarate_032.fcs · Specimen_001_RgRpR+glycolate_112.fcs · Specimen_001_RgRpR-arabinose_015.fcs · Specimen_001_RgRpR-glucarate_031.fcs · Specimen_001_RgRpR-glycolate_111.fcs · Specimen_001_RpRG10+arabinose_080.fcs · Specimen_001_RpRG10+glucarate_034.fcs · Specimen_001_RpRG10+glycolate_024.fcs · Specimen_001_RpRG10-arabinose_079.fcs · Specimen_001_RpRG10-glucarate_033.fcs · Specimen_001_RpRG10-glycolate_023.fcs · Specimen_001_RpTRg+arabinose_084.fcs · Specimen_001_RpTRg+glucarate_036.fcs · Specimen_001_RpTRg+glycolate_067.fcs · Specimen_001_RpTRg-arabinose_083.fcs · Specimen_001_RpTRg-glucarate_035.fcs · Specimen_001_RpTRg-glycolate_066.fcs · Specimen_001_V-glucarate_005.fcs |
| Timepoints | |
|---|---|
| · 200805 | 200805_RG10+GroELS(1)_001.fcs · 200805_RG10-GroELS(5)_006.fcs · 200805_RM10+GroELS(2)_039.fcs · 200805_RM10-GroELS(4)_037.fcs · 200805_RM2+GroELS(3)_016.fcs · 200805_RM2-GroELS(1)_010.fcs · 200805_RR3+GroELS(2)_023.fcs · 200805_RR3-GroELS(2)_019.fcs |
