Experiment Overview
| Repository ID: | FR-FCM-Z2V4 | Experiment name: | Treg gating frozen cord blood | MIFlowCyt score: | 65.50% |
| Primary researcher: | Richard Duggleby | PI/manager: | Richard Duggleby | Uploaded by: | Richard Duggleby |
| Experiment dates: | 2018-04-26 - 2019-04-26 | Dataset uploaded: | Aug 2020 | Last updated: | Oct 2020 |
| Keywords: | [CD3+CD4+] [Tregs] [Human cord blood] | Manuscripts: | |||
| Organizations: |
Anthony Nolan, Research, London, (UK)
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| Purpose: | Identifying Tregs in frozen umbilical cord blood segments with and without live gating | ||||
| Conclusion: | Tregs are defined as CD45+ efluro 506- CD3+CD4+CD25+CD127low FOXP3hi | ||||
| Comments: | internal gating against CD127hi FOXP3 low effector T cells | ||||
| Funding: | Not disclosed | ||||
| Quality control: | compensation controls and fluorescence-1 control | ||||
