Experiment Overview
| Repository ID: | FR-FCM-Z2WB | Experiment name: | Human CD4+ and CD8+ T Lymphocytes have Significantly Different Surface Expression Patterns of CD226 and TIGIT Molecules | MIFlowCyt score: | 79.37% |
| Primary researcher: | Marina Sunina | PI/manager: | Kai Kisand | Uploaded by: | Marina Sunina |
| Experiment dates: | 2017-04-22 - 2017-05-25 | Dataset uploaded: | Mar 2020 | Last updated: | Jul 2020 |
| Keywords: | [IL-22] [IL-17] [IFNg] [T cells] [CD28] [CD226] [TIGIT] | Manuscripts: | |||
| Organizations: |
University of Tartu, Department of Immunology, Tartu, (Estonia)
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| Purpose: | In this study, we aimed to compare pre- and post-stimulation CD226 and TIGIT expression profiles to those of CD28, in human CD4+ and CD8+ T cell subpopulations, using flow cytometry. The impact of the CD226 single nucleotide polymorphism, rs763361, on cell surface CD226 expression and effector cytokine secretion was also examined. | ||||
| Conclusion: | In this study, cell surface expression of CD226 and TIGIT, by CD4+ and CD8+ T lymphocyte subpopulations, of healthy donors before and after stimulation, was investigated. The results obtained indicate that most naïve CD4+ and CD8+ T cells did not express CD226 and TIGIT, predominantly upregulating activating receptors following stimulation. Memory CD4+ T cells exhibited balanced expression of activating and inhibitory receptors, pre- and post-stimulation. In contrast, memory CD8+ T cells predominantly expressed TIGIT. Degranulating cytotoxic T cells were characterized by high and low expression levels of activating receptor and inhibitory receptor, respectively. The rs763361 TT genotype was associated with both a reduction in CD226 expression on the cell surface of CD4+ memory T cells (p = 0.004) and increased interleukin 17A secretion from activated T cells (p = 0.036, measured from culture medium). Taken together, these results suggest a mechanism through which rs763361 can affect T cell function, creating a predisposition for autoimmune disease development. Description of different expression patterns on CD4+ and CD8+ T lymphocytes provided in this work will lead to a more comprehensive understanding of the role of the CD226/TIGIT axis in control over T cell activation and suppression. | ||||
| Comments: | Peripheral blood mononuclear cells from healthy blood donors (n = 65) were studied. | ||||
| Funding: | This study was supported by the EU Regional Developmental Fund and the Estonian Research Council grants (IUT20-43, PUT1367 and PRG 712). | ||||
| Quality control: | Cytometer Setup & Tracking Beads (BD Biosciences); Unstained cells; Fluorescence minus one controls. | ||||
