Experiment Overview
| Repository ID: | FR-FCM-Z2XA | Experiment name: | Validation of whole blood CyTOF staining using the Fluidigm MDIPA panel and SmartTube cryopreservation | MIFlowCyt score: | 60.97% |
| Primary researcher: | Daniel Geanon | PI/manager: | Adeeb Rahman | Uploaded by: | Daniel Geanon |
| Experiment dates: | 2020-03-16 - 2020-06-26 | Dataset uploaded: | Jun 2020 | Last updated: | Jul 2020 |
| Keywords: | [Immunophenotyping] [whole blood] [CyTOF] [immune-monitoring] | Manuscripts: | |||
| Organizations: |
Icahn School of Medicine at Mount Sinai , New York, NY (United States of America)
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| Purpose: | Optimization and validation of a streamlined CyTOF whole blood processing workflow, utilizing commercially available MDIPA and SmartTube reagents to facilitate multi-site CyTOF immune monitoring efforts. | ||||
| Conclusion: | Our workflow using the MDIPA panel and SmartTube fixation faithfully reproduces the conventional MDIPA workflow. | ||||
| Comments: | None | ||||
| Funding: | NIH U19AI118610, U24AI118644 and U24CA224319 | ||||
| Quality control: | Samples were acquired and normalized using Fluidigm EQ normalization beads. Samples within specific comparison groups were barcoded and pooled for acquisition to minimize experimental variation during data acquisition. | ||||
