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Purpose:
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Comparing performance of CyTOFv1, CyTOFv2, CyTOFv2-to-Helios upgrade, and two Helios instruments on aliquots of frozen prestained PBMC samples. PBMC samples from pooled vials of same donor, stained with surface antibodies, fixed with 2% PFA, permeabilized with saponin, stained with Ir intercalator, pooled again, and resplit into replicate aliquots that were then frozen at -80C in FBS+10% DMSO until use. Therefore, the samples are as identical as possible, leaving instrumentation as the main variable.
All files are in Di mode.
Compared Raw (no normalization) vs Fluidigm-ver2 normalization vs MATLAB normalization for each instrument.
v2 instrument was upgraded to create the v2-to-Helios instrument.
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