Experiment Overview
| Repository ID: | FR-FCM-Z2Y6 | Experiment name: | JCI Insights, Billi et al 2019 | MIFlowCyt score: | 79.16% |
| Primary researcher: | Brett Hill | PI/manager: | Brett Hill | Uploaded by: | Brett Hill |
| Experiment dates: | 2018-09-24 - 2019-04-03 | Dataset uploaded: | Apr 2019 | Last updated: | Apr 2019 |
| Keywords: | [mass cytometry] [autoimmunity] [Vgll3] | Manuscripts: | |||
| Organizations: |
University of Michigan, Department of Dermatology, Ann Arbor, Michigan (USA)
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| Purpose: | We recently identified VGLL3, a putative transcription factor enriched in skin of women, as a hormone-independent regulator of female-biased genes involved in autoimmunity, including factors with established roles in lupus pathogenesis. However, the local and systemic consequences of VGLL3 enrichment in female skin are unknown. Here we study skin-targeted overexpression of Vgll3 in a mouse model. To interrogate the immune landscape, we analyzed skin-draining lymph nodes, spleen, and ear tissue from WT and transgenic mice with mass cytometry (CyTOF) using a 37-parameter panel. | ||||
| Conclusion: | CyTOF data showed B cells were overrepresented among inflammatory cells in transgenic lymph nodes, with a similar although less pronounced trend in spleen. It was also found that neutrophils were enriched in transgenic ear samples. Together, these findings indicate that Vgll3 overexpression in the epidermis drives a systemic inflammatory response with B cell expansion that extends beyond the skin to the lymphatic system. | ||||
| Comments: | None | ||||
| Funding: | Work was supported by the A. Alfred Taubman Medical Research Institute Taubman Institute Innovation Projects (TIIP) program (JEG, JMK, LCT, and FW), Parfet Emerging Scholar Award (JMK), and Kenneth and Frances Eisenberg Emerging Scholar Award (JEG); the University of Michigan Babcock Endowment Fund (LCT, JEG); the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) of the National Institutes of Health (NIH) (5T32AR007197-40 to ACB; K01AR072129 to LCT; R01-AR071384 to JMK; R01-AR069071 to JEG; and P50-AR070590, R01-AR062546, R01-AR063437, and R01-AR073196 to NLW); the Office of The Director (OD) of the NIH (S10-OD020053 to FW); and the National Science Foundation (NSF) (1511720, 1645229, and 1653611 to FW). JMK is additionally supported by a Rheumatology Research Foundation Career Development K Supplement Award. LCT is additionally supported by the Dermatology Foundation and Arthritis National Research Foundation. LCT and NLW are additionally supported by the National Psoriasis Foundation. BDH, SMR, and FW are additionally partially supported by a Rogel Cancer Center Support Grant (NIH P30 CA046592). | ||||
| Quality control: | Panel staining was validated on wild-type mouse spleens. Data are representative of two independent experiments | ||||
Experiment variables
| Conditions | |
|---|---|
| · Vgll3 | 201Ear_092618_09_1.fcs · 201LN_092618_04_1.fcs · 201spleen_092618_15_0.fcs · 202Ear_092618_10_1.fcs · 202spleen_092618_16_1.fcs · 203Ear_092618_11_1.fcs · 203LN_092618_05_1.fcs · 203spleen_092618_17_1.fcs |
| · WT | 101Ear_092618_06_1.fcs · 101LN_092618_01_1.fcs · 101spleen_092618_12_0.fcs · 102Ear_092618_07_1.fcs · 102LN_092618_02_1.fcs · 102spleen_092618_13_0.fcs · 103Ear_092618_08_1.fcs · 103LN_092618_03_0.fcs · 103spleen_092618_14_0.fcs |
| Individuals | |
|---|---|
| · 101 | 101Ear_092618_06_1.fcs · 101LN_092618_01_1.fcs · 101spleen_092618_12_0.fcs |
| · 102 | 102Ear_092618_07_1.fcs · 102LN_092618_02_1.fcs · 102spleen_092618_13_0.fcs |
| · 103 | 103Ear_092618_08_1.fcs · 103LN_092618_03_0.fcs · 103spleen_092618_14_0.fcs |
| · 201 | 201Ear_092618_09_1.fcs · 201LN_092618_04_1.fcs · 201spleen_092618_15_0.fcs |
| · 202 | 202Ear_092618_10_1.fcs · 202spleen_092618_16_1.fcs |
| · 203 | 203Ear_092618_11_1.fcs · 203LN_092618_05_1.fcs · 203spleen_092618_17_1.fcs |
| Sample Type | |
|---|---|
| · Ear | 101Ear_092618_06_1.fcs · 102Ear_092618_07_1.fcs · 103Ear_092618_08_1.fcs · 201Ear_092618_09_1.fcs · 202Ear_092618_10_1.fcs · 203Ear_092618_11_1.fcs |
| · Lymph node | 101LN_092618_01_1.fcs · 102LN_092618_02_1.fcs · 103LN_092618_03_0.fcs · 201LN_092618_04_1.fcs · 203LN_092618_05_1.fcs |
| · Spleen | 101spleen_092618_12_0.fcs · 102spleen_092618_13_0.fcs · 103spleen_092618_14_0.fcs · 201spleen_092618_15_0.fcs · 202spleen_092618_16_1.fcs · 203spleen_092618_17_1.fcs |
