Experiment Overview
| Repository ID: | FR-FCM-Z2YU | Experiment name: | Immuno-flowFISH assessment of CLL with trisomy 12 or del(17p) | MIFlowCyt score: | 0.00% |
| Primary researcher: | Kathy Fuller | PI/manager: | Kathy Fuller | Uploaded by: | Kathy Fuller |
| Experiment dates: | 2014-02-03 - 2018-06-29 | Dataset uploaded: | Apr 2019 | Last updated: | Jun 2019 |
| Keywords: | [Immunophenotyping] [Imaging cytometry] [S-FISH] [haematological malignancy] [spot counting FISH analysis] [chromosomal abnormalities] [chronic lymphocytic leukaemia] [and diagnostic methodology.] | Manuscripts: | [31016848] |
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| Organizations: | None | ||||
| Purpose: | Development and application of an automated immuno-flowFISH method for the assessment of trisomy 12 and deletion 17p in chronic lymphocytic leukemia to validate its clinical utility and technical advancements over standard diagnostic methods. | ||||
| Conclusion: | Automated immuno-flowFISH provides robust diagnostic assessment of clinically relevant +12 and del(17p) CLL through specific FISH analysis in phenotypically identified cells. Specifically, spot count ratios normalized to the neoplastic CD19/CD5+ CLL population enables quantitative accuracy and precision in the detection of cytogenetic abnormalities even for low-level and atypical disease. Immuno-flowFISH has clinical utility and offers several technical advantages over standard diagnostic methods (i.e. manual slide-based FISH testing) to potentially enhance the diagnostic assessment and clinical management of CLL. | ||||
| Comments: | Imaging flow cytometry files (rif) | ||||
| Funding: | University of Western Australia Faculty of Health and Medical Research Early Career grant | ||||
| Quality control: | Simply Cellular anti-mouse compensation standard controls (Bangs Laboratories Inc., Indiana, USA) were used as fluorescence controls. Cells tested with isotype controls. Sub-samples of immunophenotyped and unstained cells were collected after staining step and analysed with samples treated with the full protocol as morphological and immunophenotype controls. All immuno-flowFISH data has been correlated with routine diagnostically compliant standard tests (i.e. standard flow cytometry and FISH). Daily instrument calibration with BD Ultra-Rainbow calibration beads and in-built software automated “ASSIST” calibration tests for instrument performance. | ||||
