Experiment Overview

Repository ID: FR-FCM-Z2ZH Experiment name: 28-colour human PBMCs MIFlowCyt score: 63.50%
Primary researcher: Cindy Ma PI/manager: Cindy Ma Uploaded by: Cindy Ma
Experiment dates: 2017-11-08 - 2018-12-12 Dataset uploaded: Mar 2019 Last updated: Mar 2019
Keywords: [Lymphocytes] [Human immunity] [primary immunodeficiency (PID)] Manuscripts: Cytalogo
Organizations: Garvan Institute of Medical Research, Darlinghurst, NSW (Australia)
Garvan Institute of Medical Research, Immunology Division, Darlinghurst, NSW (Australia)
Purpose: To develop and characterize a broad optimized fluorescent flow cytometry characterization panel for human peripheral blood mononuclear cells (PBMCs) to screen patient samples.
Conclusion: We have developed a 28 colour fluorescent characterization panel for the detection of a broad range of lymphocytes in the human.
Comments: Sample were compensated using either FACS DiVa or FlowJo using single stain controls run on the same day with identical instrument settings. Data was transformed using standard biexponential transformation settings in DiVa or Flowjo unless outliers required manual scaling to prevent data bunching.
Funding: RS is supported by the ISAC shared Resource Laboratory emerging leader program 2014-2018. CSM is supported by grants and fellowships awarded by the National Health and Medical Research Council of Australia (1138359, 1139663, 1139865, 1127157, 1124681) and the Office of Health and Medical Research of the New South Wales State Government.
Quality control: Controls included: no stain controls, Single stain controls, Florescent minus one controls, multiple PID patients and normal human controls.
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