Experiment Overview
| Repository ID: | FR-FCM-Z2ZK | Experiment name: | Comparison of the Helios narrow bore and wide bore injectors to the CyTOF2 on PBMC samples | MIFlowCyt score: | 65.75% |
| Primary researcher: | Brian Lee | PI/manager: | Adeeb Rahman | Uploaded by: | Brian Lee |
| Experiment dates: | 2018-01-22 - 2019-03-22 | Dataset uploaded: | Mar 2019 | Last updated: | Aug 2019 |
| Keywords: | [Helios] [Wide Bore Injector] [CyTOF2] | Manuscripts: | [31364278] |
|
|
| Organizations: |
Icahn School of Medicine at Mount Sinai , New York, NY (United States of America)
|
||||
| Purpose: | To evaluate the staining quality of parallel PBMC aliquots of the same sample acquired on the same Helios instrument using the original narrow bore (NB) injector and the modified wide bore (WB) injector using both H20 and Fluidigm's CAS buffer during acquisition. | ||||
| Conclusion: | Using the WB injector in conjunction with Fluidigm's CAS buffer during acquisition improves data quality collected on the Helios. When comparing the 3 configurations (NB+H20, WB+H20 and WB+CAS), the WB+CAS configuration improves data quality compared to the original NB injector. Marker medians and CVs obtained using the WB+CAS acquisition protocol were comparable or superior to the CyTOF2. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | The same stained PBMC sample was divided into multiple aliquots for acquisition on the CyTOF2 and the three Helios configurations (NB+H20, WB+H20, WB+CAS). | ||||
Experiment variables
| Sample Type | |
|---|---|
| · NB+H20 | 180130_HIMC03_B3B4_Helios_N_H2O_01_1_cells-beads.fcs · 180309_HIMC03_Helios245_NB_H2O_01_0_cells-beads.fcs · 180502_HIMC03_QC-test_NB_H20_1_cells-beads.fcs |
| · WB+H20 | 180130_HIMC03_B3B4_Helios_W_H2O.fcs_01_1_cells-beads.fcs · 180309_HIMC03_Helios245_WB_H2O_01_1_cells-beads.fcs · 180502_HIMC03_QC-REF-WATER_WB_1_cells-beads.fcs |
| · WB+CAS | 180130_HIMC03_B3B4_Helios_W_CAS_01_1_cells-beads.fcs · 180309_HIMC03_Helios245_WB_CAS_01_1_cells-beads.fcs · 180502_HIMC03_qc-ref-cas_WB_1_cells-beads.fcs · B180502_HIMC03_QC-test-ref_1_cells-beads.fcs |
| · CyTOF2 | 180130_HIMC03_B3B4_CyTOF2_H2O_1_cells-beads.fcs · 180309_HIMC03_CyTOF065-H2O_0_cells-beads.fcs · 180417_HIMC03_ref_before upgrade_1_cells-beads.fcs |
