Experiment Overview
| Repository ID: | FR-FCM-Z2ZL | Experiment name: | Comparison of the Helios wide bore and narrow bore injectors in multi-site mass cytometry studies on PBMC samples | MIFlowCyt score: | 68.00% |
| Primary researcher: | Brian Lee | PI/manager: | Adeeb Rahman | Uploaded by: | Brian Lee |
| Experiment dates: | 2018-05-01 - 2019-03-22 | Dataset uploaded: | Mar 2019 | Last updated: | Aug 2019 |
| Keywords: | [CyTOF] [multi-site study] [Helios Injector] | Manuscripts: | [31364278] |
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| Organizations: |
Icahn School of Medicine at Mount Sinai , New York, NY (United States of America)
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| Purpose: | To evaluate data quality and inter-instrument variability using the Helios wide bore injector compared to the narrow bore injector in multi-site mass cytometry studies. | ||||
| Conclusion: | The modified WB+CAS acquisition protocol reduces inter-instrument variability and improves data quality resulting in more comparable data across instruments in multi-site mass cytometry studies, | ||||
| Comments: | Relative performance improvements seen with the WB injector compared to the NB injector are both somewhat instrument and sample specific. Some Helios instruments may see a much larger change in performance and data quality than others. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | We prepared a large batch of cells comprised of a pool of CD45 barcoded PBMCs from 5 independent donors stained with the same immunophenotyping panel. Aliquots of these stained cells were distributed to 7 institutions and acquired on 10 different Helios instruments using a shared staining and sample acquisition protocol. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · NB | PS180612_SiteA_NB.fcs · PS180612_SiteB_NB.fcs · PS180612_SiteC_NB.fcs · PS180612_SiteD_NB.fcs · PS180612_SiteE_NB.fcs · PS180612_SiteF_NB.fcs · PS180612_SiteG_NB.fcs · PS180612_SiteH_NB.fcs · PS180612_SiteI_NB.fcs · PS180612_SiteJ_NB.fcs |
| · WB | PS180612_SiteA_WB.fcs · PS180612_SiteB_WB.fcs · PS180612_SiteC_WB.fcs · PS180612_SiteD_WB.fcs · PS180612_SiteE_WB.fcs · PS180612_SiteF_WB.fcs · PS180612_SiteG_WB.fcs · PS180612_SiteH_WB.fcs · PS180612_SiteI_WB.fcs · PS180612_SiteJ_WB.fcs |
| Individuals | |
|---|---|
| · SiteA | PS180612_SiteA_NB.fcs · PS180612_SiteA_WB.fcs |
| · SiteB | PS180612_SiteB_NB.fcs · PS180612_SiteB_WB.fcs |
| · SiteC | PS180612_SiteC_NB.fcs · PS180612_SiteC_WB.fcs |
| · SiteD | PS180612_SiteD_NB.fcs · PS180612_SiteD_WB.fcs |
| · SiteE | PS180612_SiteE_NB.fcs · PS180612_SiteE_WB.fcs |
| · SiteF | PS180612_SiteF_NB.fcs · PS180612_SiteF_WB.fcs |
| · SiteG | PS180612_SiteG_NB.fcs · PS180612_SiteG_WB.fcs |
| · SiteH | PS180612_SiteH_NB.fcs · PS180612_SiteH_WB.fcs |
| · SiteI | PS180612_SiteI_NB.fcs · PS180612_SiteI_WB.fcs |
| · SiteJ | PS180612_SiteJ_NB.fcs · PS180612_SiteJ_WB.fcs |
