Experiment Overview
| Repository ID: | FR-FCM-Z2ZQ | Experiment name: | Dendritic cells DAGL-beta, Vaccination | MIFlowCyt score: | 97.65% |
| Primary researcher: | Myungsun shin | PI/manager: | Ku-Lung Hsu | Uploaded by: | Myungsun shin |
| Experiment dates: | 2015-11-13 - 2015-11-20 | Dataset uploaded: | Mar 2019 | Last updated: | Mar 2019 |
| Keywords: | [CD8 T cells] [dendritic cells] [vaccination] [DAGLB] | Manuscripts: | |||
| Organizations: |
University of Virginia, Charlottesville, VA (USA)
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| Purpose: | The purpose of this experiment was to test whether the DAGL-beta inhibition in dendritic cells affects DC priming ability to T-cells. | ||||
| Conclusion: | There were no differences in DAGL-beta or alpha disruption compared to wild type dendritic cells. | ||||
| Comments: | Dendritic cells were activated and injected into Black 6 mice. After 7 days, spleens from the mice were harvested and splenocytes were analyzed for CD8T cells that are expressing the labeling with OVA-dex | ||||
| Funding: | the University of Virginia(start-up funds toK.-L.H.)and National Institutes of Health (DA035864 and DA043571 toK.-L.H.; CA166458to T.N.J.B.). | ||||
| Quality control: | Quality controls were performed by testing the experimental conditions against the dendritic cells from wild type, DAGL-beta KO, and DAGL-alpha KO mice. The same number of cells were injected and mice were allowed to remain at the same vivarium with identical conditions. Cells resulting from the analyses were subjected to the same gating strategy for quality control. | ||||
Experiment variables
| Conditions | |
|---|---|
| · Dendritic cells Activated | AKO ACTa_Tube_001_009.fcs · AKO ACTb_Tube_001_010.fcs · AKO ACTc_Tube_001_011.fcs · BKO ACTa_Tube_001_012.fcs · BKO ACTb_Tube_001_013.fcs · BKO ACTc_Tube_001_014.fcs · WT ACTa_Tube_001_006.fcs · WT ACTb_Tube_001_007.fcs · WT ACTc_Tube_001_008.fcs |
| · Dendritic cells unactivated | AKO unact a_Tube_001_018.fcs · AKO unact b_Tube_001_019.fcs · AKO unact c_Tube_001_020.fcs · BKO unact a_Tube_001_021.fcs · BKO unact b_Tube_001_022.fcs · BKO unact c_Tube_001_023.fcs · WT unact a_Tube_001_015.fcs · WT unact b_Tube_001_016.fcs · WT unact c_Tube_001_017.fcs |
| Sample Type | |
|---|---|
| · Dendritic cells | AKO ACTa_Tube_001_009.fcs · AKO ACTb_Tube_001_010.fcs · AKO ACTc_Tube_001_011.fcs · AKO unact a_Tube_001_018.fcs · AKO unact b_Tube_001_019.fcs · AKO unact c_Tube_001_020.fcs · BKO ACTa_Tube_001_012.fcs · BKO ACTb_Tube_001_013.fcs · BKO ACTc_Tube_001_014.fcs · BKO unact a_Tube_001_021.fcs · BKO unact b_Tube_001_022.fcs · BKO unact c_Tube_001_023.fcs · WT ACTa_Tube_001_006.fcs · WT ACTb_Tube_001_007.fcs · WT ACTc_Tube_001_008.fcs · WT unact a_Tube_001_015.fcs · WT unact b_Tube_001_016.fcs · WT unact c_Tube_001_017.fcs |
