Experiment Overview
| Repository ID: | FR-FCM-Z2ZV | Experiment name: | HSC-MSC quiescence | MIFlowCyt score: | 92.94% |
| Primary researcher: | Aidan Gilchrist | PI/manager: | Brendan Harley | Uploaded by: | Aidan Gilchrist |
| Experiment dates: | 2018-11-01 - | Dataset uploaded: | Mar 2019 | Last updated: | Apr 2019 |
| Keywords: | [hematopoietic stem cells] [quiescence] [mesenchymal stromal cells (MSC)] [remodeling] [artificial stem cell niche] | Manuscripts: |
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| Organizations: |
University of Illinois at Urbana-Champaign, Flow Cytometry Facility Roy J. Carver Biotechnology Center, Urbana, Illinois (United States)
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| Purpose: | Cells in 1:0 Low and 1:1 High [HSPC:MSC] conditions were cultured and hematopoietic phenotype and cell cycle analysis examined. | ||||
| Conclusion: | None | ||||
| Comments: | None | ||||
| Funding: | National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health under Award Numbers R01 DK099528 (B.A.C.H) and F31 DK117514 (A.E.G.), as well as by the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health under Award Numbers R21 EB018481 (B.A.C.H.) and T32 EB019944 (A.E.G.). | ||||
| Quality control: | Liquid experiments were performed with 3 replicates per condition Hydrogel conditions were performed with 10 replicates per condition All conditions had an unstained sample and a Live/Dead single stain sample | ||||
Experiment variables
| Conditions | |
|---|---|
| · 1:0 Low | 20181204_Low 10_012.fcs · 20181204_Low 1_003.fcs · 20181204_Low 2_004.fcs · 20181204_Low 3_005.fcs · 20181204_Low 4_006.fcs · 20181204_Low 5_007.fcs · 20181204_Low 6_008.fcs · 20181204_Low 7_009.fcs · 20181204_Low 8_010.fcs · 20181204_Low 9_011.fcs · 20181204_Low L,2f,D control_002.fcs · 20181204_Low unstained_001.fcs |
| · 1:1 High | 20181204_High 10_024.fcs · 20181204_High 11_025.fcs · 20181204_High 1_015.fcs · 20181204_High 2_016.fcs · 20181204_High 3_017.fcs · 20181204_High 4_018.fcs · 20181204_High 5_019.fcs · 20181204_High 6_020.fcs · 20181204_High 7_021.fcs · 20181204_High 8_022.fcs · 20181204_High 9_023.fcs · 20181204_High L,2f,D control_014.fcs · 20181204_High unstained_013.fcs |
| · 1:0 liquid | 20181204_L Low 1_028.fcs · 20181204_L Low 2_029.fcs · 20181204_L Low 3_030.fcs · 20181204_L Low L,2f,D control_027.fcs · 20181204_L Low unstained_026.fcs |
| · 1:1 liquid | 20181204_L High 1_033.fcs · 20181204_L High 2_034.fcs · 20181204_L High 3_035.fcs · 20181204_L High L,2f,D control_032.fcs · 20181204_L High unstained_031.fcs |
