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Experiment Overview

Repository ID: FR-FCM-Z32M Experiment name: Human Fetal Liver CD34+ population deep phenotyping by spectral flow cytometry MIFlowCyt score: 65.75%
Primary researcher: Anna Belkina PI/manager: Anna Belkina Uploaded by: Anna Belkina
Experiment dates: 2020-10-18 - 2020-10-18 Dataset uploaded: Oct 2020 Last updated: Jun 2022
Keywords: [CD34] [hematopoietic stem cells] [Immunophenotyping] [spectral data] Manuscripts: [35232959] [PMC8888592]
Organizations: Boston University School of Medicine, Flow Cytometry Core Facility, Boston, MA (USA)
Purpose: Characterization of CD34+ hematopoietic progenitor population in human fetal liver
Conclusion: Our data illustrate the heterogeneity of the CD34+ compartment of the human fetal liver
Comments: Vanuytsel K, Villacorta-Martin C, Lindstrom-Vautrin J, Wang Z, Garcia-Beltran WF, Vrbanac V, Parsons D, Lam EC, Matte TM, Dowrey TW, Kumar SS, Li M, Wang F, Yeung AK, Mostoslavsky G, Dries R, Campbell JD, Belkina AC, Balazs AB, Murphy GJ. Multi-modal profiling of human fetal liver hematopoietic stem cells reveals the molecular signature of engraftment. Nat Commun. 2022 Mar 1;13(1):1103. doi: 10.1038/s41467-022-28616-x. PubMed PMID: 35232959; PubMed Central PMCID: PMC8888592.
Funding: Internal
Quality control: Instrument QC, reference control run as a separate sample, fluorescence minus multiple control


Experiment variables

Conditions
· Fetal liver full stain FL 1.fcs · full stain FL 2.fcs · full stain FL 3.fcs · full stain FL 4.fcs · full stain FL 5.fcs
· PBMC full stain PBMC.fcs

Sample Type
· Fetal liver full stain FL 1.fcs · full stain FL 2.fcs · full stain FL 3.fcs · full stain FL 4.fcs · full stain FL 5.fcs
· PBMC full stain PBMC.fcs

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