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Experiment: HAP1 WT and MED13KO MMS - AnnexinV apoptosis
ID: FR-FCM-Z32T
Primary Researcher: Nicola Pietro Montaldo
MIFlowCyt Score: 23.50%

Experiment Overview

Repository ID:	FR-FCM-Z32T	Experiment name:	HAP1 WT and MED13KO MMS - AnnexinV apoptosis	MIFlowCyt score:	23.50%
Primary researcher:	Nicola Pietro Montaldo	PI/manager:	Nicola Pietro Montaldo	Uploaded by:	Nicola Pietro Montaldo
Experiment dates:	2020-08-01 - 2020-09-30	Dataset uploaded:	Oct 2020	Last updated:	Dec 2020
Keywords:	None		Manuscripts:
Organizations:	None
Purpose:	Flow cytometric determination of apoptosis by annexin V/propidium iodide double staining after MMS treatment (125 uM 72h)
Conclusion:	upon MMS treatment MED13 KO cells show a reduced apoptotic population in comparison to the WT
Comments:	None
Funding:	Not disclosed
Quality control:	- annexin V single staining - propidium iodide single staining sample - no staining sample

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The following open access article describes how to upload and annotate flow cytometry data sets: Spidlen J, Breuer K and Brinkman R. Preparing a Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) Compliant Manuscript Using the International Society for Advancement of Cytometry (ISAC) FCS File Repository (FlowRepository.org). Current Protocols in Cytometry, UNIT 10.18, July 2012.
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You may download slides from our Workshop at CYTO 2012: Publishing MIFlowCyt Compliant Data to ISAC’s FlowRepository.org for Cytometry A and Other Journals
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Citing FlowRepository

Please reference us by citing:

Spidlen J, Breuer K, Rosenberg C, Kotecha N and Brinkman RR. FlowRepository - A Resource of Annotated Flow Cytometry Datasets Associated with Peer-reviewed Publications. Cytometry A. 2012 Sep; 81(9):727-31..

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