Experiment Overview
| Repository ID: | FR-FCM-Z333 | Experiment name: | Inhibition of uracil removal by ATA in MCF7 cells | MIFlowCyt score: | 46.61% |
| Primary researcher: | Daniel Laverty | PI/manager: | Zachary Nagel | Uploaded by: | Daniel Laverty |
| Experiment dates: | 2020-08-29 - 2020-09-13 | Dataset uploaded: | Oct 2020 | Last updated: | Apr 2024 |
| Keywords: | [DNA Repair] | Manuscripts: | [29122935] | ||
| Organizations: |
Harvard T.H. Chan School of Public Health, Boston, MA (United States)
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| Purpose: | Testing the inhibition of uracil removal from DNA by the inhibitor ATA. | ||||
| Conclusion: | ATA inhibits the removal of uracil from a plasmid based assay in MCF7 cells | ||||
| Comments: | MCF7 cells were seeded into plates and treated with DMSO or with a putative uracil dna glycosylase inhibitor (ATA, sometimes referred to as ATA mix). Following treatment, cells were transfected with a plasmid-based reporter that measures uracil removal. The samples labelled "Undamaged" were transfected with plasmids encoding BFP and GFP. The samples labeled "Damaged" were transfected with a plasmid encoding GFP and a uracil-containing plasmid encoding BFP. If uracil is present in the plasmid, wild type BFP transcripts are produced. If uracil is removed. non-fluorescent transcripts are produced. Hence, increased BFP fluorescence in the samples labeled "Damaged" means decreased removal of uracil from DNA. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Daily performance tests on the flow cytometer using the manufacturer's performance tracking beads. | ||||
