Experiment Overview
| Repository ID: | FR-FCM-Z34A | Experiment name: | Kinetics of osmotic stress regulate a cell fate switch of cell survival-Ramp10h-ID-92019-Jurkat cells exposed to hypertonicity | MIFlowCyt score: | 18.00% |
| Primary researcher: | Gregor Neuert | PI/manager: | Gregor Neuert | Uploaded by: | Gregor Neuert |
| Experiment dates: | 2020-11-08 - | Dataset uploaded: | Nov 2020 | Last updated: | Jan 2021 |
| Keywords: | None | Manuscripts: | |||
| Organizations: | None | ||||
| Purpose: | To understand the molecular changes of signaling and markers of cell state when cells are exposed to hypertonic stress, we measured time courses for the below markers: comb ramp10 combined comb ramp10 FSC_H comb ramp10 FSC_W comb ramp10 FSC_A comb ramp10 SSC_H comb ramp10 SSC_W comb ramp10 SSC_A combaba ramp10 SC_MKP1_FITC combaba ramp10 BD_pS6_A647 combaba ramp10 SC_NFAT5_A594 combabb ramp10 CS_aCaspase8_PE combabb ramp10 BD_aCaspase3_A647 combabc ramp10 BD_aCaspase3_PE combabc ramp10 BD_cPARP_A647 combabd ramp10 CS_pJNK_A647 combabd ramp10 CS_pMKK4_PE combabe ramp10 BD_IFNy_A700 combabe ramp10 BD_IL1b_PE combabf ramp10 BD_pH2AX_A555 combabf ramp10 BD_Ki67_A700 combabg ramp10 BD_pH2AX_A555 combabg ramp10 BD_FAS_PECy5 combabh ramp10 BD_pCREB_A647 combabh ramp10 BD_pmTOR_PE combabk ramp10 CS_p27kip1_PE combabk ramp10 CS_pHsp27_A647 combabi ramp10 CS_CHOP_A647 combabi ramp10 CS_LC3_PE combabj ramp10 CS_aCaspase9_PE combabj ramp10 BD_pNfkb_A647 combabp ramp10 CS_PP2AC_PE combabp ramp10 CS_pp38_A700 combabl ramp10 CS_p38_PE combabl ramp10 SC_Caspase12_A647 combabm ramp10 CS_pBad_PE combabm ramp10 BD_pMK2_A647 combabn ramp10 BD_FAS_PECy5 combabn ramp10 BD_FASL_PE combabo ramp10 BD_Bcl2_PE combabo ramp10 CS_BclXL_A647 combabq ramp10 SC_NOXA_A594 combabq ramp10 SC_ppp2ac_A680 combabr ramp10 RD_NLRP3_A700 combabr ramp10 CS_pATF2_PE combabs ramp10 SC_Bax_A647 combabs ramp10 CS_pIKKab_PE combabt ramp10 CS_pStat3_A647 combabt ramp10 CS_LC3b_PE combabw ramp10 BD_TNF_A700 combabw ramp10 CS_pcJun_PE combabz4 ramp10 BD_pZAP70_A647 | ||||
| Conclusion: | Conclusions are described in: https://www.biorxiv.org/content/10.1101/2020.07.10.197871v1 | ||||
| Comments: | timepoints 0,48,77,109,134,154,189,268,379,464,535,600 min after addition of 300 mosmol/l NaCl are barcoded by 12 barcodes using Pacific-Blue (dilution: high, mid, low) and Pacific-Orange ((dilution: high, midhigh, midlow, low) following established protocols for fluorescent cell barcoding for flow cytometry: timepoints are barcoded as follows: PO high + PB high=0, PO midhigh + PB high=48, PO midlow + PB high=77, PO low + PB high=109, PO high + PB mid=134, PO midhigh + PB mid=154, PO midlow + PB mid=189, PO low + PB mid=268, PO high + PB low=379, PO midhigh + PB low=464, PO midlow + PB low=535, PO low + PB low=600 | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
