Experiment Overview
| Repository ID: | FR-FCM-Z34E | Experiment name: | Kinetics of osmotic stress regulate a cell fate switch of cell survival-Ramp10h_5h -ID-110320-Jurkat cells exposed to hypertonicity | MIFlowCyt score: | 18.00% |
| Primary researcher: | Gregor Neuert | PI/manager: | Gregor Neuert | Uploaded by: | Gregor Neuert |
| Experiment dates: | 2020-11-08 - | Dataset uploaded: | Nov 2020 | Last updated: | Jan 2021 |
| Keywords: | None | Manuscripts: | |||
| Organizations: | None | ||||
| Purpose: | To understand the molecular changes of signaling and markers of cell state when cells are exposed to hypertonic stress, we measured time courses for the below markers: comb ramp10_5h combined comb ramp10_5h FSC_H comb ramp10_5h FSC_W comb ramp10_5h FSC_A comb ramp10_5h SSC_H comb ramp10_5h SSC_W comb ramp10_5h SSC_A combabb ramp10_5h CS_aCaspase8_PE combabb ramp10_5h BD_aCaspase3_A647 combaba ramp10_5h BD_pS6_A647 combaba ramp10_5h SC_NFAT5_A594 combabd ramp10_5h BD_pH2AX_A555 combabd ramp10_5h BD_Ki67_A700 combabe ramp10_5h BD_pNfkb_A647 combabe ramp10_5h CS_p38_PE combabg ramp10_5h BD_cPARP_A647 combabg ramp10_5h CS_aCaspase9_PE combabf ramp10_5h CS_pp38_A594 combabj ramp10_5h CS_pp38_A594 combabj ramp10_5h BD_pH3_A647 combabh ramp10_5h CS_pATF2_7_PE combabh ramp10_5h SC_SGK1_A680 combabi ramp10_5h BD_pRb_A647 combabi ramp10_5h CS_p27kip1_PE combabk ramp10_5h BD_aCaspase3_PE combabk ramp10_5h CS_CHOP_A647 | ||||
| Conclusion: | Conclusions are described in: https://www.biorxiv.org/content/10.1101/2020.07.10.197871v1 | ||||
| Comments: | timepoints 0,600,630,660,690,720,750,780,810,840,900 min after addition of 300 mosmol/l NaCl are barcoded by 12 barcodes using Pacific-Blue (dilution: high, mid, low) and Pacific-Orange ((dilution: high, midhigh, midlow, low) following established protocols for fluorescent cell barcoding for flow cytometry: timepoints are barcoded as follows: PO high + PB high=0, PO midhigh + PB high=600, PO midlow + PB high=630, PO low + PB high=660, PO high + PB mid=690, PO midhigh + PB mid=720, PO midlow + PB mid=750, PO low + PB mid=780, PO high + PB low=810, PO midhigh + PB low=840, PO midlow + PB low=870, PO low + PB low=900 | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
