Experiment Overview
| Repository ID: | FR-FCM-Z34F | Experiment name: | Kinetics of osmotic stress regulate a cell fate switch of cell survival-Step200 -ID-101320-Jurkat cells exposed to hypertonicity | MIFlowCyt score: | 18.00% |
| Primary researcher: | Gregor Neuert | PI/manager: | Gregor Neuert | Uploaded by: | Gregor Neuert |
| Experiment dates: | 2020-11-08 - | Dataset uploaded: | Nov 2020 | Last updated: | Jan 2021 |
| Keywords: | None | Manuscripts: | |||
| Organizations: | None | ||||
| Purpose: | To understand the molecular changes of signaling and markers of cell state when cells are exposed to hypertonic stress, we measured time courses for the below markers: comb step200 combined comb step200 FSC_H comb step200 FSC_W comb step200 FSC_A comb step200 SSC_H comb step200 SSC_W comb step200 SSC_A combabb step200 CS_aCaspase8_PE combabb step200 BD_aCaspase3_A647 combaba step200 BD_pS6_A647 combaba step200 SC_NFAT5_A594 combabc step200 BD_aCaspase3_PE combabc step200 BD_cPARP_A647 combabd step200 BD_pH2AX_A555 combabd step200 BD_Ki67_A700 combabe step200 BD_pNfkb_A647 combabf step200 CS_pp38_A594 combabf step200 SC_pASK_A680 | ||||
| Conclusion: | Conclusions are described in: https://www.biorxiv.org/content/10.1101/2020.07.10.197871v1 | ||||
| Comments: | timepoints 0,2,5,10,15,20,30,60,120,180,240 300 min after addition of 300 mosmol/l NaCl are barcoded by 12 barcodes using Pacific-Blue (dilution: high, mid, low) and Pacific-Orange ((dilution: high, midhigh, midlow, low) following established protocols for fluorescent cell barcoding for flow cytometry: timepoints are barcoded as follows: PO high + PB high=0, PO midhigh + PB high=2, PO midlow + PB high=5, PO low + PB high=10, PO high + PB mid=15, PO midhigh + PB mid=20, PO midlow + PB mid=30, PO low + PB mid=60, PO high + PB low=120, PO midhigh + PB low=180, PO midlow + PB low=240, PO low + PB low=300 | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
