Experiment Overview
| Repository ID: | FR-FCM-Z34R | Experiment name: | Intracellular calcium levels and mitochondrial membrane potential in chilled boar spermatozoa | MIFlowCyt score: | 53.75% |
| Primary researcher: | Helen Jaekel | PI/manager: | Dagmar Waberski | Uploaded by: | Helen Jaekel |
| Experiment dates: | 2018-06-20 - 2018-07-09 | Dataset uploaded: | Nov 2020 | Last updated: | Nov 2020 |
| Keywords: | [multicolor] [boar spermatozoa] [chilling injury] [MMP] [Calcium] | Manuscripts: | |||
| Organizations: |
University of Veterinary Medicine Hannover, Unit for Reproductive Medicine, Hannover, (Germany)
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| Purpose: | Identification of sublethal chilling injuries in boar spermatozoa using multicolor flow cytometry. Intracellular calcium levels and mitochondrial membrane potential were assessed. | ||||
| Conclusion: | Sublethal chilling injuries could be detected with multicolor flow cytometry | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | unstained samples, basic settings and induced response with calcium ionophore | ||||
Experiment variables
| Timepoints | |
|---|---|
| · 3min | Boar 1_17°C_CM_3min.fcs · Boar 1_17°C_NCM_3min.fcs · Boar 1_5°C_CM_3min.fcs · Boar 1_5°C_NCM_3min.fcs · Boar 2_17°C_CM_3min.fcs · Boar 2_17°C_NCM_3min.fcs · Boar 2_5°C_CM_3min.fcs · Boar 2_5°C_NCM_3min.fcs · Boar 3_17°C_CM_3min.fcs · Boar 3_17°C_NCM_3min.fcs · Boar 3_5°C_CM_3min.fcs · Boar 3_5°C_NCM_3min.fcs |
| · 60min | Boar 1_17°C_CM_60min.fcs · Boar 1_17°C_CM_60min_CaIono.fcs · Boar 1_17°C_NCM_60min.fcs · Boar 1_17°C_NCM_60min_CaIono.fcs · Boar 1_5°C_CM_60min.fcs · Boar 1_5°C_CM_60min_CaIono.fcs · Boar 1_5°C_NCM_60min.fcs · Boar 1_5°C_NCM_60min_CaIono.fcs · Boar 2_17°C_CM_60min.fcs · Boar 2_17°C_CM_60min_CaIono.fcs · Boar 2_17°C_NCM_60min.fcs · Boar 2_17°C_NCM_60min_CaIono.fcs · Boar 2_5°C_CM_60min.fcs · Boar 2_5°C_CM_60min_CaIono.fcs · Boar 2_5°C_NCM_60min.fcs · Boar 2_5°C_NCM_60min_CaIono.fcs · Boar 3_17°C_CM_60min.fcs · Boar 3_17°C_CM_60min_CaIono.fcs · Boar 3_17°C_NCM_60min.fcs · Boar 3_17°C_NCM_60min_CaIono.fcs · Boar 3_5°C_CM_60min.fcs · Boar 3_5°C_CM_60min_CaIono.fcs · Boar 3_5°C_NCM_60min.fcs · Boar 3_5°C_NCM_60min_CaIono.fcs |
| Conditions | |
|---|---|
| · CM | Boar 1_17°C_CM_3min.fcs · Boar 1_17°C_CM_60min.fcs · Boar 1_17°C_CM_60min_CaIono.fcs · Boar 1_5°C_CM_3min.fcs · Boar 1_5°C_CM_60min.fcs · Boar 1_5°C_CM_60min_CaIono.fcs · Boar 2_17°C_CM_3min.fcs · Boar 2_17°C_CM_60min.fcs · Boar 2_17°C_CM_60min_CaIono.fcs · Boar 2_5°C_CM_3min.fcs · Boar 2_5°C_CM_60min.fcs · Boar 2_5°C_CM_60min_CaIono.fcs · Boar 3_17°C_CM_3min.fcs · Boar 3_17°C_CM_60min.fcs · Boar 3_17°C_CM_60min_CaIono.fcs · Boar 3_5°C_CM_3min.fcs · Boar 3_5°C_CM_60min.fcs · Boar 3_5°C_CM_60min_CaIono.fcs |
| · NCM | Boar 1_17°C_NCM_3min.fcs · Boar 1_17°C_NCM_60min.fcs · Boar 1_17°C_NCM_60min_CaIono.fcs · Boar 1_5°C_NCM_3min.fcs · Boar 1_5°C_NCM_60min.fcs · Boar 1_5°C_NCM_60min_CaIono.fcs · Boar 2_17°C_NCM_3min.fcs · Boar 2_17°C_NCM_60min.fcs · Boar 2_17°C_NCM_60min_CaIono.fcs · Boar 2_5°C_NCM_3min.fcs · Boar 2_5°C_NCM_60min.fcs · Boar 2_5°C_NCM_60min_CaIono.fcs · Boar 3_17°C_NCM_3min.fcs · Boar 3_17°C_NCM_60min.fcs · Boar 3_17°C_NCM_60min_CaIono.fcs · Boar 3_5°C_NCM_3min.fcs · Boar 3_5°C_NCM_60min.fcs · Boar 3_5°C_NCM_60min_CaIono.fcs |
| Individuals | |
|---|---|
| · Boar 1 | Boar 1_17°C_CM_3min.fcs · Boar 1_17°C_CM_60min.fcs · Boar 1_17°C_CM_60min_CaIono.fcs · Boar 1_17°C_NCM_3min.fcs · Boar 1_17°C_NCM_60min.fcs · Boar 1_17°C_NCM_60min_CaIono.fcs · Boar 1_5°C_CM_3min.fcs · Boar 1_5°C_CM_60min.fcs · Boar 1_5°C_CM_60min_CaIono.fcs · Boar 1_5°C_NCM_3min.fcs · Boar 1_5°C_NCM_60min.fcs · Boar 1_5°C_NCM_60min_CaIono.fcs |
| · Boar 2 | Boar 2_17°C_CM_3min.fcs · Boar 2_17°C_CM_60min.fcs · Boar 2_17°C_CM_60min_CaIono.fcs · Boar 2_17°C_NCM_3min.fcs · Boar 2_17°C_NCM_60min.fcs · Boar 2_17°C_NCM_60min_CaIono.fcs · Boar 2_5°C_CM_3min.fcs · Boar 2_5°C_CM_60min.fcs · Boar 2_5°C_CM_60min_CaIono.fcs · Boar 2_5°C_NCM_3min.fcs · Boar 2_5°C_NCM_60min.fcs · Boar 2_5°C_NCM_60min_CaIono.fcs |
| · Boar 3 | Boar 3_17°C_CM_3min.fcs · Boar 3_17°C_CM_60min.fcs · Boar 3_17°C_CM_60min_CaIono.fcs · Boar 3_17°C_NCM_3min.fcs · Boar 3_17°C_NCM_60min.fcs · Boar 3_17°C_NCM_60min_CaIono.fcs · Boar 3_5°C_CM_3min.fcs · Boar 3_5°C_CM_60min.fcs · Boar 3_5°C_CM_60min_CaIono.fcs · Boar 3_5°C_NCM_3min.fcs · Boar 3_5°C_NCM_60min.fcs · Boar 3_5°C_NCM_60min_CaIono.fcs |
| Sample Type | |
|---|---|
| · 5°C | Boar 1_5°C_CM_3min.fcs · Boar 1_5°C_CM_60min.fcs · Boar 1_5°C_CM_60min_CaIono.fcs · Boar 1_5°C_NCM_3min.fcs · Boar 1_5°C_NCM_60min.fcs · Boar 1_5°C_NCM_60min_CaIono.fcs · Boar 2_5°C_CM_3min.fcs · Boar 2_5°C_CM_60min.fcs · Boar 2_5°C_CM_60min_CaIono.fcs · Boar 2_5°C_NCM_3min.fcs · Boar 2_5°C_NCM_60min.fcs · Boar 2_5°C_NCM_60min_CaIono.fcs · Boar 3_5°C_CM_3min.fcs · Boar 3_5°C_CM_60min.fcs · Boar 3_5°C_CM_60min_CaIono.fcs · Boar 3_5°C_NCM_3min.fcs · Boar 3_5°C_NCM_60min.fcs · Boar 3_5°C_NCM_60min_CaIono.fcs |
| · 17°C | Boar 1_17°C_CM_3min.fcs · Boar 1_17°C_CM_60min.fcs · Boar 1_17°C_CM_60min_CaIono.fcs · Boar 1_17°C_NCM_3min.fcs · Boar 1_17°C_NCM_60min.fcs · Boar 1_17°C_NCM_60min_CaIono.fcs · Boar 2_17°C_CM_3min.fcs · Boar 2_17°C_CM_60min.fcs · Boar 2_17°C_CM_60min_CaIono.fcs · Boar 2_17°C_NCM_3min.fcs · Boar 2_17°C_NCM_60min.fcs · Boar 2_17°C_NCM_60min_CaIono.fcs · Boar 3_17°C_CM_3min.fcs · Boar 3_17°C_CM_60min.fcs · Boar 3_17°C_CM_60min_CaIono.fcs · Boar 3_17°C_NCM_3min.fcs · Boar 3_17°C_NCM_60min.fcs · Boar 3_17°C_NCM_60min_CaIono.fcs |
