Experiment Overview
| Repository ID: | FR-FCM-Z34U | Experiment name: | Mapping the recovery of critically ill COVID 19 patients by mass cytometry | MIFlowCyt score: | 79.59% |
| Primary researcher: | Pier Andree Penttila | PI/manager: | Pier Andree Penttila | Uploaded by: | Pier Andree Penttila |
| Experiment dates: | 2020-03-30 - 2020-05-26 | Dataset uploaded: | Nov 2020 | Last updated: | Dec 2020 |
| Keywords: | [human] [mass cytometry] [CyTOF] [immune-monitoring] [COVID19] [cytokine multiplexing] | Manuscripts: | |||
| Organizations: |
KU Leuven, Leuven, Vlaams Brabant (Belgium)
|
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| Purpose: | Here, we have analysed longitudinally collected, whole blood samples of 40 surviving COVID-19 patients during their recovery at ICU using high-dimensional cytometry by time-of-flight (CyTOF) and cytokine multiplexing. | ||||
| Conclusion: | We defined 4 sequential immunotypes during recovery that correlated to various clinical parameters, including the level of respiratory support at concomitant sampling times. We also identified classical monocytes as the first immune cell type to recover by restoring HLA-DR-positivity and by reducing the immunosuppressive CD163+ monocyte population, followed by the recovery of CD8+ and CD4+ T cell, and mDC populations. The identified immunotypes also correlated to aberrant cytokine and acute-phase reactant levels. Finally, integrative analysis of cytokines and immune cell profiles showed a shift from an initially dysregulated immune response to a more coordinated immunogenic interplay, highlighting the importance of longitudinal sampling to understand the pathophysiology underlying recovery from severe COVID-19. | ||||
| Comments: | None | ||||
| Funding: | This work was supported by FWO grant I007418N, KULeuven grant C14/17/084, KOOR funding and ‘het Leuven Kankerinstituut’. S.V.G. is an ISAC Marylou Ingram Scholar and supported by an FWO postdoctoral research grant (Research Foundation – Flanders). L. V. is supported by an FWO PhD fellowship (Research Foundation – Flanders, E11E9819N). | ||||
| Quality control: | 1. Antibodies not included in the Fluidigm MaxPar Direct Immune Profiling kit were titirated on RBC lysed whole blood to determine the optimal concentration. 2. The cryopreservation technique was validated in triplicate by dividing aliquots of donor samples stained on the same day and comparing cell viability and immune profiles between the fresh and cryopreserved samples. 3. Batch effects were evaluated by running a reference sample derived from an aliquot of the same healthy donor over the period of the study. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · Ward Admission | COVID_W_027_O_LiHep_00_1_cleaned.fcs · COVID_W_031_O_LiHep_00_1_cleaned.fcs · COVID_W_036_A_LiHep_00_1_cleaned.fcs · COVID_W_036_O_LiHep_00_1_cleaned.fcs · Covid_W_027_A_LiHep_00_1_cleaned.fcs · Covid_W_039_A_LiHep_00_1_cleaned.fcs · Covid_W_049_O_LiHep_00_1_cleaned.fcs |
| · ICU Admission | COVID_ICU_023_A_LiHep_00_1_cleaned.fcs · COVID_ICU_027_A_LiHep_00_1_cleaned.fcs · COVID_ICU_029_A_LiHep_00_1_cleaned.fcs · COVID_ICU_031_A_LiHep_02_1_cleaned.fcs · COVID_ICU_036_A_LiHep_00_1_cleaned.fcs · COVID_ICU_039_A_LiHep_00_1_cleaned.fcs · COVID_ICU_043_A_LiHep_00_1_cleaned.fcs · COVID_ICU_050_A_LiHep_00_1_cleaned.fcs · COVID_ICU_051_A_LiHep_00_1_cleaned.fcs · Covid_ICU_013_Admission_WB1_01_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_014_Admission_WB2_02_1_Ungated_1.fcs_cleaned.fcs · Covid_ICU_014_Admission_WB2_02_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_015_Admission_WB3_03_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_016_Admission_WB4_04_1_Ungated_1.fcs_cleaned.fcs · Covid_ICU_016_Admission_WB4_04_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_024_A_01_1_cleaned.fcs · Covid_ICU_044_A_LiHep_00_1_cleaned.fcs · Covid_ICU_045_A_LiHep_00_1_cleaned.fcs · Covid_ICU_047_A_00_1_cleaned.fcs · Covid_ICU_048_A_LiHep_00_1_cleaned.fcs · Covid_ICU_057_A_LiHep_00_1_cleaned.fcs |
| · ICU midpoint | COVID_ICU_009_Z_LiHep_01_1_cleaned.fcs · COVID_ICU_013_C_LiHep_00_1_cleaned.fcs · COVID_ICU_013_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_014_C_LiHep_00_1_cleaned.fcs · COVID_ICU_014_Y_LiHep_01_1_cleaned.fcs · COVID_ICU_015_C_LiHep_00_1_cleaned.fcs · COVID_ICU_019_C_LiHep_00_1_cleaned.fcs · COVID_ICU_020_C_LiHep_00_1_cleaned.fcs · COVID_ICU_021_C_LiHep_00_1_cleaned.fcs · COVID_ICU_021_Z_LiHep_01_1_cleaned.fcs · COVID_ICU_022_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_023_C_LiHep_00_1_cleaned.fcs · COVID_ICU_023_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_026_C_LiHep_00_1_cleaned.fcs · COVID_ICU_026_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_028_Z_LiHep_01_1_cleaned.fcs · COVID_ICU_029_Y_LiHep_00_1_cleaned.fcs · COVID_ICU_034_C_LiHep_00_1_cleaned.fcs · COVID_ICU_035_C_LiHep_00_1_cleaned.fcs · COVID_ICU_037_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_038_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_040_C_LiHep_00_1_cleaned.fcs · COVID_ICU_041_C_LiHep_00_1_cleaned.fcs · COVID_ICU_044_E1_LiHep_00_1_cleaned.fcs · COVID_ICU_044_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_048_C_LiHep_00_1_cleaned.fcs · COVID_ICU_050_C_LiHep_00_1_cleaned.fcs · COVID_ICU_050_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_051_C_LiHep_00_1_cleaned.fcs · COVID_ICU_054_C_LiHep_01_1_cleaned.fcs · COVID_ICU_057_L2_LiHep_00_1_cleaned.fcs · COVID_W_049_C_LiHep_00_2_cleaned.fcs · Covid_ICU_009_E1_LiHep_00_1_cleaned.fcs · Covid_ICU_014_2_00_1_Ungated_1.fcs_cleaned.fcs · Covid_ICU_021_E1_LiHep_00_1_cleaned.fcs · Covid_ICU_027_C_LiHep_00_1_cleaned.fcs · Covid_ICU_031_C_LiHep_00_1_cleaned.fcs · Covid_ICU_038_E1_LiHep_00_1_cleaned.fcs · Covid_ICU_039_C_LiHep_00_1_cleaned.fcs · Covid_ICU_042_Z_LiHep_00_1_cleaned.fcs · Covid_ICU_043_C_LiHep_00_1_cleaned.fcs · Covid_ICU_044_C_LiHep_00_1_cleaned.fcs · Covid_ICU_044_Y_00_1_cleaned.fcs · Covid_ICU_045_C_LiHep_00_1_cleaned.fcs · Covid_ICU_046_Z_LiHep_00_1_cleaned.fcs · Covid_ICU_057_B_LiHep_00_1_cleaned.fcs · Covid_ICU_057_C_LiHep_00_1_cleaned.fcs · Covid_ICU_057_L1_LiHep_00_1_cleaned.fcs · Covid_W_027_C_LiHep_00_1_cleaned.fcs · Covid_W_031_C_LiHep_00_1_cleaned (1).fcs · Covid_W_039_C_LiHep_00_1_cleaned.fcs |
| · ICU discharge | COVID_ICU_015_D_LiHep_00_1_cleaned.fcs · COVID_ICU_019_D_LiHep_00_1_cleaned.fcs · COVID_ICU_020_D_LiHep_00_1_cleaned.fcs · COVID_ICU_021_D_LiHep_00_1_cleaned.fcs · COVID_ICU_023_D_LiHep_00_1_cleaned.fcs · COVID_ICU_024_D_LiHep_00_1_cleaned.fcs · COVID_ICU_027_D_LiHep_00_1_cleaned (2).fcs · COVID_ICU_028_D_LiHep_00_1_cleaned (1).fcs · COVID_ICU_031_D_LiHep_00_1_cleaned.fcs · COVID_ICU_037_D_LiHep_00_1_cleaned.fcs · COVID_ICU_039_D_LiHep_00_1_cleaned.fcs · COVID_ICU_040_D_LiHep_00_1_cleaned.fcs · COVID_ICU_043_D_LiHep_00_1_cleaned.fcs · COVID_ICU_045_D_LiHep_00_1_cleaned.fcs · COVID_ICU_046_D_LiHep_00_1_cleaned.fcs · COVID_ICU_047_D_00_1_cleaned.fcs · COVID_ICU_048_D_LiHep_01_1_cleaned.fcs · COVID_ICU_050_D_LiHep_00_1_cleaned.fcs · COVID_ICU_051_D_LiHep_00_1_cleaned.fcs · COVID_ICU_054_D_LiHep_00_1_cleaned.fcs · COVID_W_027_D_LiHep_00_1_cleaned.fcs · COVID_W_031_D_LiHep_00_1_cleaned.fcs · COVID_W_036_D_LiHep_00_1_cleaned.fcs · COVID_W_039_D_LiHep_00_1_cleaned.fcs · COVID_W_049_D_LiHep_00_1_cleaned.fcs · Covid_ICU_009_D_00_1_cleaned.fcs · Covid_ICU_013_D_00_1_cleaned.fcs · Covid_ICU_014_D_LiHep_00_1_cleaned.fcs · Covid_ICU_016_D_LiHep_00_1_Ungated_1.fcs_cleaned.fcs · Covid_ICU_022_D_LiHep_00_1_cleaned.fcs · Covid_ICU_026_D_00_1_cleaned.fcs · Covid_ICU_029_D_00_1_cleaned.fcs · Covid_ICU_034_D_00_1_cleaned.fcs · Covid_ICU_035_D_00_1_cleaned.fcs · Covid_ICU_036_D_00_1_cleaned.fcs · Covid_ICU_038_D_00_1_cleaned.fcs · Covid_ICU_041_D_LiHep_00_1_cleaned.fcs · Covid_ICU_042_D_00_1_cleaned.fcs · Covid_ICU_044_D_00_1_cleaned.fcs |
| · Healthy Controls | Covid_HC_001_01_1_cleaned.fcs · Covid_HC_003_01_1_cleaned.fcs · Covid_HC_004_01_1_cleaned.fcs · Covid_HC_005_01_1_cleaned (1).fcs · Covid_HC_006_00_1_cleaned.fcs · Covid_HC_007_00_1_cleaned.fcs · Covid_HC_008_00_1_cleaned.fcs · Covid_HC_009_00_1_cleaned.fcs |
| Sample Type | |
|---|---|
| · Human whole blood | COVID_ICU_009_Z_LiHep_01_1_cleaned.fcs · COVID_ICU_013_C_LiHep_00_1_cleaned.fcs · COVID_ICU_013_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_014_C_LiHep_00_1_cleaned.fcs · COVID_ICU_014_Y_LiHep_01_1_cleaned.fcs · COVID_ICU_015_C_LiHep_00_1_cleaned.fcs · COVID_ICU_015_D_LiHep_00_1_cleaned.fcs · COVID_ICU_019_C_LiHep_00_1_cleaned.fcs · COVID_ICU_019_D_LiHep_00_1_cleaned.fcs · COVID_ICU_020_C_LiHep_00_1_cleaned.fcs · COVID_ICU_020_D_LiHep_00_1_cleaned.fcs · COVID_ICU_021_C_LiHep_00_1_cleaned.fcs · COVID_ICU_021_D_LiHep_00_1_cleaned.fcs · COVID_ICU_021_Z_LiHep_01_1_cleaned.fcs · COVID_ICU_022_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_023_A_LiHep_00_1_cleaned.fcs · COVID_ICU_023_C_LiHep_00_1_cleaned.fcs · COVID_ICU_023_D_LiHep_00_1_cleaned.fcs · COVID_ICU_023_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_024_D_LiHep_00_1_cleaned.fcs · COVID_ICU_026_C_LiHep_00_1_cleaned.fcs · COVID_ICU_026_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_027_A_LiHep_00_1_cleaned.fcs · COVID_ICU_027_D_LiHep_00_1_cleaned (2).fcs · COVID_ICU_028_D_LiHep_00_1_cleaned (1).fcs · COVID_ICU_028_Z_LiHep_01_1_cleaned.fcs · COVID_ICU_029_A_LiHep_00_1_cleaned.fcs · COVID_ICU_029_Y_LiHep_00_1_cleaned.fcs · COVID_ICU_031_A_LiHep_02_1_cleaned.fcs · COVID_ICU_031_D_LiHep_00_1_cleaned.fcs · COVID_ICU_034_C_LiHep_00_1_cleaned.fcs · COVID_ICU_035_C_LiHep_00_1_cleaned.fcs · COVID_ICU_036_A_LiHep_00_1_cleaned.fcs · COVID_ICU_037_D_LiHep_00_1_cleaned.fcs · COVID_ICU_037_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_038_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_039_A_LiHep_00_1_cleaned.fcs · COVID_ICU_039_D_LiHep_00_1_cleaned.fcs · COVID_ICU_040_C_LiHep_00_1_cleaned.fcs · COVID_ICU_040_D_LiHep_00_1_cleaned.fcs · COVID_ICU_041_C_LiHep_00_1_cleaned.fcs · COVID_ICU_043_A_LiHep_00_1_cleaned.fcs · COVID_ICU_043_D_LiHep_00_1_cleaned.fcs · COVID_ICU_044_E1_LiHep_00_1_cleaned.fcs · COVID_ICU_044_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_045_D_LiHep_00_1_cleaned.fcs · COVID_ICU_046_D_LiHep_00_1_cleaned.fcs · COVID_ICU_047_D_00_1_cleaned.fcs · COVID_ICU_048_C_LiHep_00_1_cleaned.fcs · COVID_ICU_048_D_LiHep_01_1_cleaned.fcs · COVID_ICU_050_A_LiHep_00_1_cleaned.fcs · COVID_ICU_050_C_LiHep_00_1_cleaned.fcs · COVID_ICU_050_D_LiHep_00_1_cleaned.fcs · COVID_ICU_050_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_051_A_LiHep_00_1_cleaned.fcs · COVID_ICU_051_C_LiHep_00_1_cleaned.fcs · COVID_ICU_051_D_LiHep_00_1_cleaned.fcs · COVID_ICU_054_C_LiHep_01_1_cleaned.fcs · COVID_ICU_054_D_LiHep_00_1_cleaned.fcs · COVID_ICU_057_L2_LiHep_00_1_cleaned.fcs · COVID_W_027_D_LiHep_00_1_cleaned.fcs · COVID_W_027_O_LiHep_00_1_cleaned.fcs · COVID_W_031_D_LiHep_00_1_cleaned.fcs · COVID_W_031_O_LiHep_00_1_cleaned.fcs · COVID_W_036_A_LiHep_00_1_cleaned.fcs · COVID_W_036_D_LiHep_00_1_cleaned.fcs · COVID_W_036_O_LiHep_00_1_cleaned.fcs · COVID_W_039_D_LiHep_00_1_cleaned.fcs · COVID_W_049_C_LiHep_00_2_cleaned.fcs · COVID_W_049_D_LiHep_00_1_cleaned.fcs · Covid_HC_001_01_1_cleaned.fcs · Covid_HC_003_01_1_cleaned.fcs · Covid_HC_004_01_1_cleaned.fcs · Covid_HC_005_01_1_cleaned (1).fcs · Covid_HC_006_00_1_cleaned.fcs · Covid_HC_007_00_1_cleaned.fcs · Covid_HC_008_00_1_cleaned.fcs · Covid_HC_009_00_1_cleaned.fcs · Covid_ICU_009_D_00_1_cleaned.fcs · Covid_ICU_009_E1_LiHep_00_1_cleaned.fcs · Covid_ICU_013_Admission_WB1_01_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_013_D_00_1_cleaned.fcs · Covid_ICU_014_2_00_1_Ungated_1.fcs_cleaned.fcs · Covid_ICU_014_Admission_WB2_02_1_Ungated_1.fcs_cleaned.fcs · Covid_ICU_014_Admission_WB2_02_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_014_D_LiHep_00_1_cleaned.fcs · Covid_ICU_015_Admission_WB3_03_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_016_Admission_WB4_04_1_Ungated_1.fcs_cleaned.fcs · Covid_ICU_016_Admission_WB4_04_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_016_D_LiHep_00_1_Ungated_1.fcs_cleaned.fcs · Covid_ICU_021_E1_LiHep_00_1_cleaned.fcs · Covid_ICU_022_D_LiHep_00_1_cleaned.fcs · Covid_ICU_024_A_01_1_cleaned.fcs · Covid_ICU_026_D_00_1_cleaned.fcs · Covid_ICU_027_C_LiHep_00_1_cleaned.fcs · Covid_ICU_029_D_00_1_cleaned.fcs · Covid_ICU_031_C_LiHep_00_1_cleaned.fcs · Covid_ICU_034_D_00_1_cleaned.fcs · Covid_ICU_035_D_00_1_cleaned.fcs · Covid_ICU_036_D_00_1_cleaned.fcs · Covid_ICU_038_D_00_1_cleaned.fcs · Covid_ICU_038_E1_LiHep_00_1_cleaned.fcs · Covid_ICU_039_C_LiHep_00_1_cleaned.fcs · Covid_ICU_041_D_LiHep_00_1_cleaned.fcs · Covid_ICU_042_D_00_1_cleaned.fcs · Covid_ICU_042_Z_LiHep_00_1_cleaned.fcs · Covid_ICU_043_C_LiHep_00_1_cleaned.fcs · Covid_ICU_044_A_LiHep_00_1_cleaned.fcs · Covid_ICU_044_C_LiHep_00_1_cleaned.fcs · Covid_ICU_044_D_00_1_cleaned.fcs · Covid_ICU_044_Y_00_1_cleaned.fcs · Covid_ICU_045_A_LiHep_00_1_cleaned.fcs · Covid_ICU_045_C_LiHep_00_1_cleaned.fcs · Covid_ICU_046_Z_LiHep_00_1_cleaned.fcs · Covid_ICU_047_A_00_1_cleaned.fcs · Covid_ICU_048_A_LiHep_00_1_cleaned.fcs · Covid_ICU_057_A_LiHep_00_1_cleaned.fcs · Covid_ICU_057_B_LiHep_00_1_cleaned.fcs · Covid_ICU_057_C_LiHep_00_1_cleaned.fcs · Covid_ICU_057_L1_LiHep_00_1_cleaned.fcs · Covid_W_027_A_LiHep_00_1_cleaned.fcs · Covid_W_027_C_LiHep_00_1_cleaned.fcs · Covid_W_031_C_LiHep_00_1_cleaned (1).fcs · Covid_W_039_A_LiHep_00_1_cleaned.fcs · Covid_W_039_C_LiHep_00_1_cleaned.fcs · Covid_W_049_O_LiHep_00_1_cleaned.fcs |
| Individuals | |
|---|---|
| · Female | COVID_ICU_014_C_LiHep_00_1_cleaned.fcs · COVID_ICU_014_Y_LiHep_01_1_cleaned.fcs · COVID_ICU_020_C_LiHep_00_1_cleaned.fcs · COVID_ICU_020_D_LiHep_00_1_cleaned.fcs · COVID_ICU_024_D_LiHep_00_1_cleaned.fcs · COVID_ICU_036_A_LiHep_00_1_cleaned.fcs · COVID_ICU_038_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_043_A_LiHep_00_1_cleaned.fcs · COVID_ICU_043_D_LiHep_00_1_cleaned.fcs · COVID_ICU_047_D_00_1_cleaned.fcs · COVID_ICU_054_C_LiHep_01_1_cleaned.fcs · COVID_ICU_054_D_LiHep_00_1_cleaned.fcs · COVID_W_039_D_LiHep_00_1_cleaned.fcs · Covid_ICU_014_2_00_1_Ungated_1.fcs_cleaned.fcs · Covid_ICU_014_Admission_WB2_02_1_Ungated_1.fcs_cleaned.fcs · Covid_ICU_014_Admission_WB2_02_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_014_D_LiHep_00_1_cleaned.fcs · Covid_ICU_024_A_01_1_cleaned.fcs · Covid_ICU_036_D_00_1_cleaned.fcs · Covid_ICU_038_D_00_1_cleaned.fcs · Covid_ICU_038_E1_LiHep_00_1_cleaned.fcs · Covid_ICU_043_C_LiHep_00_1_cleaned.fcs · Covid_ICU_047_A_00_1_cleaned.fcs · Covid_W_039_A_LiHep_00_1_cleaned.fcs · Covid_W_039_C_LiHep_00_1_cleaned.fcs |
| · Male | COVID_ICU_009_Z_LiHep_01_1_cleaned.fcs · COVID_ICU_013_C_LiHep_00_1_cleaned.fcs · COVID_ICU_013_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_015_C_LiHep_00_1_cleaned.fcs · COVID_ICU_015_D_LiHep_00_1_cleaned.fcs · COVID_ICU_019_C_LiHep_00_1_cleaned.fcs · COVID_ICU_019_D_LiHep_00_1_cleaned.fcs · COVID_ICU_021_C_LiHep_00_1_cleaned.fcs · COVID_ICU_021_D_LiHep_00_1_cleaned.fcs · COVID_ICU_021_Z_LiHep_01_1_cleaned.fcs · COVID_ICU_022_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_023_A_LiHep_00_1_cleaned.fcs · COVID_ICU_023_C_LiHep_00_1_cleaned.fcs · COVID_ICU_023_D_LiHep_00_1_cleaned.fcs · COVID_ICU_023_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_026_C_LiHep_00_1_cleaned.fcs · COVID_ICU_026_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_027_A_LiHep_00_1_cleaned.fcs · COVID_ICU_027_D_LiHep_00_1_cleaned (2).fcs · COVID_ICU_028_D_LiHep_00_1_cleaned (1).fcs · COVID_ICU_028_Z_LiHep_01_1_cleaned.fcs · COVID_ICU_029_A_LiHep_00_1_cleaned.fcs · COVID_ICU_029_Y_LiHep_00_1_cleaned.fcs · COVID_ICU_031_A_LiHep_02_1_cleaned.fcs · COVID_ICU_031_D_LiHep_00_1_cleaned.fcs · COVID_ICU_034_C_LiHep_00_1_cleaned.fcs · COVID_ICU_035_C_LiHep_00_1_cleaned.fcs · COVID_ICU_037_D_LiHep_00_1_cleaned.fcs · COVID_ICU_037_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_039_A_LiHep_00_1_cleaned.fcs · COVID_ICU_039_D_LiHep_00_1_cleaned.fcs · COVID_ICU_040_C_LiHep_00_1_cleaned.fcs · COVID_ICU_040_D_LiHep_00_1_cleaned.fcs · COVID_ICU_041_C_LiHep_00_1_cleaned.fcs · COVID_ICU_044_E1_LiHep_00_1_cleaned.fcs · COVID_ICU_044_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_045_D_LiHep_00_1_cleaned.fcs · COVID_ICU_046_D_LiHep_00_1_cleaned.fcs · COVID_ICU_048_C_LiHep_00_1_cleaned.fcs · COVID_ICU_048_D_LiHep_01_1_cleaned.fcs · COVID_ICU_050_A_LiHep_00_1_cleaned.fcs · COVID_ICU_050_C_LiHep_00_1_cleaned.fcs · COVID_ICU_050_D_LiHep_00_1_cleaned.fcs · COVID_ICU_050_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_051_A_LiHep_00_1_cleaned.fcs · COVID_ICU_051_C_LiHep_00_1_cleaned.fcs · COVID_ICU_051_D_LiHep_00_1_cleaned.fcs · COVID_ICU_057_L2_LiHep_00_1_cleaned.fcs · COVID_W_027_D_LiHep_00_1_cleaned.fcs · COVID_W_027_O_LiHep_00_1_cleaned.fcs · COVID_W_031_D_LiHep_00_1_cleaned.fcs · COVID_W_031_O_LiHep_00_1_cleaned.fcs · COVID_W_036_A_LiHep_00_1_cleaned.fcs · COVID_W_036_D_LiHep_00_1_cleaned.fcs · COVID_W_036_O_LiHep_00_1_cleaned.fcs · COVID_W_049_C_LiHep_00_2_cleaned.fcs · COVID_W_049_D_LiHep_00_1_cleaned.fcs · Covid_ICU_009_D_00_1_cleaned.fcs · Covid_ICU_009_E1_LiHep_00_1_cleaned.fcs · Covid_ICU_013_Admission_WB1_01_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_013_D_00_1_cleaned.fcs · Covid_ICU_015_Admission_WB3_03_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_016_Admission_WB4_04_1_Ungated_1.fcs_cleaned.fcs · Covid_ICU_016_Admission_WB4_04_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_016_D_LiHep_00_1_Ungated_1.fcs_cleaned.fcs · Covid_ICU_021_E1_LiHep_00_1_cleaned.fcs · Covid_ICU_022_D_LiHep_00_1_cleaned.fcs · Covid_ICU_026_D_00_1_cleaned.fcs · Covid_ICU_027_C_LiHep_00_1_cleaned.fcs · Covid_ICU_029_D_00_1_cleaned.fcs · Covid_ICU_031_C_LiHep_00_1_cleaned.fcs · Covid_ICU_034_D_00_1_cleaned.fcs · Covid_ICU_035_D_00_1_cleaned.fcs · Covid_ICU_039_C_LiHep_00_1_cleaned.fcs · Covid_ICU_041_D_LiHep_00_1_cleaned.fcs · Covid_ICU_042_D_00_1_cleaned.fcs · Covid_ICU_042_Z_LiHep_00_1_cleaned.fcs · Covid_ICU_044_A_LiHep_00_1_cleaned.fcs · Covid_ICU_044_C_LiHep_00_1_cleaned.fcs · Covid_ICU_044_D_00_1_cleaned.fcs · Covid_ICU_044_Y_00_1_cleaned.fcs · Covid_ICU_045_A_LiHep_00_1_cleaned.fcs · Covid_ICU_045_C_LiHep_00_1_cleaned.fcs · Covid_ICU_046_Z_LiHep_00_1_cleaned.fcs · Covid_ICU_048_A_LiHep_00_1_cleaned.fcs · Covid_ICU_057_A_LiHep_00_1_cleaned.fcs · Covid_ICU_057_B_LiHep_00_1_cleaned.fcs · Covid_ICU_057_C_LiHep_00_1_cleaned.fcs · Covid_ICU_057_L1_LiHep_00_1_cleaned.fcs · Covid_W_027_A_LiHep_00_1_cleaned.fcs · Covid_W_027_C_LiHep_00_1_cleaned.fcs · Covid_W_031_C_LiHep_00_1_cleaned (1).fcs · Covid_W_049_O_LiHep_00_1_cleaned.fcs |
| · Healthy control female | Covid_HC_003_01_1_cleaned.fcs · Covid_HC_004_01_1_cleaned.fcs · Covid_HC_005_01_1_cleaned (1).fcs · Covid_HC_007_00_1_cleaned.fcs · Covid_HC_009_00_1_cleaned.fcs |
| · Healthy control male | Covid_HC_001_01_1_cleaned.fcs · Covid_HC_006_00_1_cleaned.fcs · Covid_HC_008_00_1_cleaned.fcs |
| Conditions | |
|---|---|
| · Healthy Controls | Covid_HC_001_01_1_cleaned.fcs · Covid_HC_003_01_1_cleaned.fcs · Covid_HC_004_01_1_cleaned.fcs · Covid_HC_005_01_1_cleaned (1).fcs · Covid_HC_006_00_1_cleaned.fcs · Covid_HC_007_00_1_cleaned.fcs · Covid_HC_008_00_1_cleaned.fcs · Covid_HC_009_00_1_cleaned.fcs |
| · Methylprednisolone (MP) treatment | COVID_ICU_013_C_LiHep_00_1_cleaned.fcs · COVID_ICU_013_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_014_C_LiHep_00_1_cleaned.fcs · COVID_ICU_014_Y_LiHep_01_1_cleaned.fcs · COVID_ICU_015_C_LiHep_00_1_cleaned.fcs · COVID_ICU_015_D_LiHep_00_1_cleaned.fcs · COVID_ICU_019_C_LiHep_00_1_cleaned.fcs · COVID_ICU_019_D_LiHep_00_1_cleaned.fcs · COVID_ICU_020_C_LiHep_00_1_cleaned.fcs · COVID_ICU_020_D_LiHep_00_1_cleaned.fcs · COVID_ICU_021_C_LiHep_00_1_cleaned.fcs · COVID_ICU_021_D_LiHep_00_1_cleaned.fcs · COVID_ICU_021_Z_LiHep_01_1_cleaned.fcs · COVID_ICU_023_A_LiHep_00_1_cleaned.fcs · COVID_ICU_023_C_LiHep_00_1_cleaned.fcs · COVID_ICU_023_D_LiHep_00_1_cleaned.fcs · COVID_ICU_023_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_026_C_LiHep_00_1_cleaned.fcs · COVID_ICU_026_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_028_D_LiHep_00_1_cleaned (1).fcs · COVID_ICU_028_Z_LiHep_01_1_cleaned.fcs · COVID_ICU_038_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_039_A_LiHep_00_1_cleaned.fcs · COVID_ICU_039_D_LiHep_00_1_cleaned.fcs · COVID_ICU_040_C_LiHep_00_1_cleaned.fcs · COVID_ICU_040_D_LiHep_00_1_cleaned.fcs · COVID_ICU_044_E1_LiHep_00_1_cleaned.fcs · COVID_ICU_044_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_045_D_LiHep_00_1_cleaned.fcs · COVID_ICU_046_D_LiHep_00_1_cleaned.fcs · COVID_ICU_054_C_LiHep_01_1_cleaned.fcs · COVID_ICU_054_D_LiHep_00_1_cleaned.fcs · COVID_W_036_A_LiHep_00_1_cleaned.fcs · COVID_W_036_D_LiHep_00_1_cleaned.fcs · COVID_W_036_O_LiHep_00_1_cleaned.fcs · COVID_W_039_D_LiHep_00_1_cleaned.fcs · Covid_ICU_013_Admission_WB1_01_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_013_D_00_1_cleaned.fcs · Covid_ICU_014_2_00_1_Ungated_1.fcs_cleaned.fcs · Covid_ICU_014_Admission_WB2_02_1_Ungated_1.fcs_cleaned.fcs · Covid_ICU_014_Admission_WB2_02_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_014_D_LiHep_00_1_cleaned.fcs · Covid_ICU_015_Admission_WB3_03_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_021_E1_LiHep_00_1_cleaned.fcs · Covid_ICU_026_D_00_1_cleaned.fcs · Covid_ICU_038_D_00_1_cleaned.fcs · Covid_ICU_038_E1_LiHep_00_1_cleaned.fcs · Covid_ICU_039_C_LiHep_00_1_cleaned.fcs · Covid_ICU_042_D_00_1_cleaned.fcs · Covid_ICU_042_Z_LiHep_00_1_cleaned.fcs · Covid_ICU_044_A_LiHep_00_1_cleaned.fcs · Covid_ICU_044_C_LiHep_00_1_cleaned.fcs · Covid_ICU_044_D_00_1_cleaned.fcs · Covid_ICU_044_Y_00_1_cleaned.fcs · Covid_ICU_045_A_LiHep_00_1_cleaned.fcs · Covid_ICU_045_C_LiHep_00_1_cleaned.fcs · Covid_ICU_046_Z_LiHep_00_1_cleaned.fcs · Covid_W_039_A_LiHep_00_1_cleaned.fcs · Covid_W_039_C_LiHep_00_1_cleaned.fcs |
| · MP + Anti-IL-6 treatment | COVID_ICU_009_Z_LiHep_01_1_cleaned.fcs · COVID_ICU_050_A_LiHep_00_1_cleaned.fcs · COVID_ICU_050_C_LiHep_00_1_cleaned.fcs · COVID_ICU_050_D_LiHep_00_1_cleaned.fcs · COVID_ICU_050_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_051_A_LiHep_00_1_cleaned.fcs · COVID_ICU_051_C_LiHep_00_1_cleaned.fcs · COVID_ICU_051_D_LiHep_00_1_cleaned.fcs · COVID_W_027_D_LiHep_00_1_cleaned.fcs · COVID_W_027_O_LiHep_00_1_cleaned.fcs · COVID_W_049_C_LiHep_00_2_cleaned.fcs · COVID_W_049_D_LiHep_00_1_cleaned.fcs · Covid_ICU_009_D_00_1_cleaned.fcs · Covid_ICU_009_E1_LiHep_00_1_cleaned.fcs · Covid_W_027_A_LiHep_00_1_cleaned.fcs · Covid_W_027_C_LiHep_00_1_cleaned.fcs · Covid_W_049_O_LiHep_00_1_cleaned.fcs |
| · MP + Anti-IL-1 treatment | COVID_ICU_027_A_LiHep_00_1_cleaned.fcs · COVID_ICU_027_D_LiHep_00_1_cleaned (2).fcs · COVID_ICU_043_A_LiHep_00_1_cleaned.fcs · COVID_ICU_043_D_LiHep_00_1_cleaned.fcs · COVID_ICU_048_C_LiHep_00_1_cleaned.fcs · COVID_ICU_048_D_LiHep_01_1_cleaned.fcs · Covid_ICU_027_C_LiHep_00_1_cleaned.fcs · Covid_ICU_043_C_LiHep_00_1_cleaned.fcs · Covid_ICU_048_A_LiHep_00_1_cleaned.fcs |
| · Anti-IL-6 treatment | Covid_ICU_016_Admission_WB4_04_1_Ungated_1.fcs_cleaned.fcs · Covid_ICU_016_Admission_WB4_04_1_Ungated_1.fcs_cleaned_cleaned.fcs · Covid_ICU_016_D_LiHep_00_1_Ungated_1.fcs_cleaned.fcs |
| · No immuno-modulatory treatment (supportive care only) | COVID_ICU_022_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_024_D_LiHep_00_1_cleaned.fcs · COVID_ICU_029_A_LiHep_00_1_cleaned.fcs · COVID_ICU_029_Y_LiHep_00_1_cleaned.fcs · COVID_ICU_031_A_LiHep_02_1_cleaned.fcs · COVID_ICU_031_D_LiHep_00_1_cleaned.fcs · COVID_ICU_034_C_LiHep_00_1_cleaned.fcs · COVID_ICU_035_C_LiHep_00_1_cleaned.fcs · COVID_ICU_036_A_LiHep_00_1_cleaned.fcs · COVID_ICU_037_D_LiHep_00_1_cleaned.fcs · COVID_ICU_037_Z_LiHep_00_1_cleaned.fcs · COVID_ICU_041_C_LiHep_00_1_cleaned.fcs · COVID_ICU_047_D_00_1_cleaned.fcs · COVID_ICU_057_L2_LiHep_00_1_cleaned.fcs · COVID_W_031_D_LiHep_00_1_cleaned.fcs · COVID_W_031_O_LiHep_00_1_cleaned.fcs · Covid_ICU_022_D_LiHep_00_1_cleaned.fcs · Covid_ICU_024_A_01_1_cleaned.fcs · Covid_ICU_029_D_00_1_cleaned.fcs · Covid_ICU_031_C_LiHep_00_1_cleaned.fcs · Covid_ICU_034_D_00_1_cleaned.fcs · Covid_ICU_035_D_00_1_cleaned.fcs · Covid_ICU_036_D_00_1_cleaned.fcs · Covid_ICU_041_D_LiHep_00_1_cleaned.fcs · Covid_ICU_047_A_00_1_cleaned.fcs · Covid_ICU_057_A_LiHep_00_1_cleaned.fcs · Covid_ICU_057_B_LiHep_00_1_cleaned.fcs · Covid_ICU_057_C_LiHep_00_1_cleaned.fcs · Covid_ICU_057_L1_LiHep_00_1_cleaned.fcs · Covid_W_031_C_LiHep_00_1_cleaned (1).fcs |
