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Experiment: CyTOF characterization of primary NPM1 mutant FLT3-ITD positive and negative AML cases
ID: FR-FCM-Z36E
Primary Researcher: Mark Gower
MIFlowCyt Score: 22.50%

Experiment Overview

Repository ID:	FR-FCM-Z36E	Experiment name:	CyTOF characterization of primary NPM1 mutant FLT3-ITD positive and negative AML cases	MIFlowCyt score:	22.50%
Primary researcher:	Mark Gower	PI/manager:	Cynthia Guidos	Uploaded by:	Mark Gower
Experiment dates:	2017-07-24 - 2017-07-27	Dataset uploaded:	Dec 2020	Last updated:	Dec 2020
Keywords:	[Acute Myeloid Leukemia]		Manuscripts:
Organizations:	Hospital for Sick Children Research Institute, Developmental & Stem Cell Biology, Toronto, ON (Canada)
Purpose:	To interrogate potential marker expression differences between cells from primary NPM1 mutant/ FLT3-ITD+ and NPM1 mutant/FLT3-ITD negative AML peripheral blood samples by mass cytometry.
Conclusion:	None
Comments:	None
Funding:	Not disclosed
Quality control:	None

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Help

The following open access article describes how to upload and annotate flow cytometry data sets: Spidlen J, Breuer K and Brinkman R. Preparing a Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) Compliant Manuscript Using the International Society for Advancement of Cytometry (ISAC) FCS File Repository (FlowRepository.org). Current Protocols in Cytometry, UNIT 10.18, July 2012.
We also have a Quick start guide and a FAQ section.
You may download slides from our Workshop at CYTO 2012: Publishing MIFlowCyt Compliant Data to ISAC’s FlowRepository.org for Cytometry A and Other Journals
Additional links and help options are listed in our support page.
You can contact us for support regarding technical issues or by sending comments about how to make your experiment or FlowRepository in general better. Leave feedback or ask questions by filling out a support ticket.

Citing FlowRepository

Please reference us by citing:

Spidlen J, Breuer K, Rosenberg C, Kotecha N and Brinkman RR. FlowRepository - A Resource of Annotated Flow Cytometry Datasets Associated with Peer-reviewed Publications. Cytometry A. 2012 Sep; 81(9):727-31..

Supporting journal

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FCS files
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Attachments
- Gating_strategy.pdf
- README.txt
- all_samples_C1vC2_cluster_abundance_counts_flowSOM-k-25_MG_20190709.txt
- all_samples_C1vC2_cluster_median_counts_flowSOM-k-25_MG_20190709.txt
- protocol_for_cytof-data-generation_and_analysis.docx