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Notice: Temporary Shutdown of Flow Repository
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Experiment Overview

Repository ID: FR-FCM-Z388 Experiment name: Sputum_Sort_Live_Dead MIFlowCyt score: 84.84%
Primary researcher: Lydia Bederka PI/manager: Vivienne Rebel Uploaded by: Lydia Bederka
Experiment dates: 2018-08-01 - 2020-12-18 Dataset uploaded: Dec 2020 Last updated: Jun 2021
Keywords: [Leukocytes] [sputum] [squamous epithelial cells] Manuscripts: Plosonelogo
Organizations: UTHealth San Antonio Flow Cytometry Core Facility, San Antonio , TX (USA)
Purpose: Identify cellular populations isolated from sputum of persons with at least a 30 pack-year history of cigarette smoking.
Conclusion: Squamous epithelial cells are the major cell population sorted from the dead cell gate, having stained brightly with the viability dye Fixable Viability Stain 510 (FVS510). The cells identified from sorting of the live cell gate showed great cellular diversity.
Comments: None
Funding: Not disclosed
Quality control: BD™ FACSAria™ platform was checked prior to each analysis session with BD FACSDiva™ CS&T Research beads. A small portion of the unstained and sort samples were used to define the sorting gates.


Experiment variables

Sample Type
· Specimen_001_LOW_BEC CD45 SORT_010 Specimen_001_LOW BEC CD45 SORT_010.fcs · Specimen_001_UNST LOW BEC_005.fcs

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