Experiment Overview
| Repository ID: | FR-FCM-Z388 | Experiment name: | Sputum_Sort_Live_Dead | MIFlowCyt score: | 84.84% |
| Primary researcher: | Lydia Bederka | PI/manager: | Vivienne Rebel | Uploaded by: | Lydia Bederka |
| Experiment dates: | 2018-08-01 - 2020-12-18 | Dataset uploaded: | Dec 2020 | Last updated: | Jun 2021 |
| Keywords: | [Leukocytes] [sputum] [squamous epithelial cells] | Manuscripts: |
|
||
| Organizations: |
UTHealth San Antonio Flow Cytometry Core Facility, San Antonio , TX (USA)
|
||||
| Purpose: | Identify cellular populations isolated from sputum of persons with at least a 30 pack-year history of cigarette smoking. | ||||
| Conclusion: | Squamous epithelial cells are the major cell population sorted from the dead cell gate, having stained brightly with the viability dye Fixable Viability Stain 510 (FVS510). The cells identified from sorting of the live cell gate showed great cellular diversity. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | BD™ FACSAria™ platform was checked prior to each analysis session with BD FACSDiva™ CS&T Research beads. A small portion of the unstained and sort samples were used to define the sorting gates. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · Specimen_001_LOW_BEC CD45 SORT_010 | Specimen_001_LOW BEC CD45 SORT_010.fcs · Specimen_001_UNST LOW BEC_005.fcs |
