Experiment Overview
| Repository ID: | FR-FCM-Z389 | Experiment name: | Sputum_Sort_CD45+_populations | MIFlowCyt score: | 87.98% |
| Primary researcher: | Lydia Bederka | PI/manager: | Vivienne Rebel | Uploaded by: | Lydia Bederka |
| Experiment dates: | 2018-08-08 - 2020-12-18 | Dataset uploaded: | Dec 2020 | Last updated: | Jun 2021 |
| Keywords: | [flow cytometry] [Leukocytes] [macrophage] [sputum] [alveolar] [diagnostics] | Manuscripts: |
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| Organizations: |
UTHealth San Antonio Flow Cytometry Core Facility, San Antonio , TX (USA)
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| Purpose: | Identify subpopulations of CD45 positive cells isolated from sputum of persons with at least a 30 pack-year history of cigarette smoking. Identifying macrophages allowed us to establish a quality control parameter to confirm the sputum sample originated from the lung and did not contain only saliva. | ||||
| Conclusion: | Alveolar and interstitial macrophages were identified from two independent populations sorted from live, CD45 positive parent populations. The presence of alveolar macrophages confirmed the lung origin of these representative samples. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | BD™ FACSAria™ platform was checked prior to each analysis session with BD FACSDiva™ CS&T Research beads. PMTs were adjusted using small amounts of the unstained, isotype, and sort samples to define the hierarchy and gates for sorted population. | ||||
