Experiment Overview
| Repository ID: | FR-FCM-Z38A | Experiment name: | Sputum_FMO | MIFlowCyt score: | 84.61% |
| Primary researcher: | Lydia Bederka | PI/manager: | Vivienne Rebel | Uploaded by: | Lydia Bederka |
| Experiment dates: | 2020-09-24 - 2020-12-18 | Dataset uploaded: | Dec 2020 | Last updated: | Dec 2020 |
| Keywords: | [flow cytometry] [High Throughput] [Leukocytes] [macrophage] [sputum] [alveolar] [interstitial] [diagnostics] | Manuscripts: |
|
||
| Organizations: |
UTHealth San Antonio Flow Cytometry Core Facility, San Antonio , TX (USA)
|
||||
| Purpose: | Identify main populations of sputum-derived cells by comparing the fluorescent profiles produced by isotype antibodies versus the fluorescent minus one (FMO) parameter. | ||||
| Conclusion: | FMO profiles produced more defined subpopulation boundaries when compared to isotype antibody profiles, allowing for cleaner cell population gate definitions. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | The LSR II cytometer platforms were checked prior to each analysis session with BD FACSDiva™ CS&T Research beads. PMTs are adjusted and checked prior to each use using SPHERO™ Rainbow Calibration Particles. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · Blood | 2020-09-25_JR_LB_FMO_Specimen_001_200153_Blood_020.fcs · 2020-09-25_JR_LB_FMO_Specimen_001_200153_Blood_Control_1_021.fcs · 2020-09-25_JR_LB_FMO_Specimen_001_200153_Blood_Control_2_022.fcs · 2020-09-25_JR_LB_FMO_Specimen_001_200153_Blood_Control_3_023.fcs |
