Experiment Overview
| Repository ID: | FR-FCM-Z38N | Experiment name: | Phenotypic analysis of IgG and IgA subclasses on human B cells | MIFlowCyt score: | 51.50% |
| Primary researcher: | Rosemarie Mason | PI/manager: | Rosemarie Mason | Uploaded by: | Rosemarie Mason |
| Experiment dates: | 2017-06-27 - 2020-12-21 | Dataset uploaded: | Dec 2020 | Last updated: | Dec 2020 |
| Keywords: | [Human preterm cord blood] [multicolor flow cytometry] [B cell] [immunoglobulin] [isotype] [subclass] | Manuscripts: | [33939254] [PMC8453800] |
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| Organizations: |
Vaccine Research Center, National Institute of Health, Bethesda, , MD, (USA)
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| Purpose: | This 18-color panel was designed for extensive phenotyping of B cell immunoglobulin isotypes and subclasses in human peripheral blood samples. | ||||
| Conclusion: | This OMIP allows for phenotyping of B cell immunoglobulin isotypes (IgD, IgM, IgA and IgG), subclasses (IgA1, IgA2, IgG1, IgG2, IgG3, IgG4) and light chain (IgKappa and IgLambda) usage. Additional markers (CD19, CD20, CD21 and CD27) allow for identification of naive B cells, memory B cell subsets, and plasmablasts. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | According to the MiFlowCyt document from the OMIP publication | ||||
