Experiment Overview
| Repository ID: | FR-FCM-Z3EX | Experiment name: | Cell cycle profiles of CCNC or CDK8 WT and KO cells | MIFlowCyt score: | 93.42% |
| Primary researcher: | Rebecca Lloyd | PI/manager: | Steve Jackson | Uploaded by: | Rebecca Lloyd |
| Experiment dates: | 2019-02-13 - 2019-08-01 | Dataset uploaded: | Jan 2021 | Last updated: | Jan 2021 |
| Keywords: | [cell cycle] [CCNC] [CDK8] | Manuscripts: | |||
| Organizations: |
University of Cambridge, Cambridge, (UK)
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| Purpose: | To assess the impact of Cyclin C or CDK8 loss on cell cycle distributions | ||||
| Conclusion: | No discernible impact of Cyclin C or CDK8 loss | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Individuals | |
|---|---|
| · WT HAP1 | WT DMSO.fcs · WT DMSO.fcs-(1) · WT DMSO.fcs-(2) |
| · CDK8 KO HAP1 (A17) | A17 DMSO.fcs · A17 DMSO.fcs-(1) · A17 DMSO.fcs-(2) |
| · CDK8 KO HAP1 (B24) | B24 DMSO.fcs · B24 DMSO.fcs-(1) · B24 DMSO.fcs-(2) |
| · WT U2-OS | U2OS WT DMSO.fcs · WT DMSO.fcs-(3) · WT DMSO.fcs-(4) |
| · CCNC KO U2-OS (A10) | U2OS A10 DMSO.fcs · A10 DMSO.fcs · A10 DMSO.fcs-(1) |
| · CCNC KO U2-OS (A11) | U2OS A11 DMSO.fcs · A11 DMSO.fcs · A11 DMSO.fcs-(1) |
| · WT mESC | 0h WT.fcs · WT DMSO.fcs-(5) · WT Origin firing DMSO EdU.fcs · mESC WT DMSO.fcs |
| · Ccnc KO mESC (D1) | 0h CCNC D1.fcs · D1 DMSO.fcs · D1 Origin firing DMSO EdU.fcs · mESC D1 DMSO.fcs |
| · Ccnc KO mESC (G2) | 0h CCNC G2.fcs · G2 DMSO.fcs · G2 origin firing DMSO EdU.fcs · mESC G2 DMSO.fcs |
| · Cdk8 KO mESC (B9) | 0h CDK8 B9.fcs · B9 DMSO.fcs · B9 origin firing DMSO EdU.fcs · mESC B9 DMSO.fcs |
| · Cdk8 KO mESC (H4) | 0h CDK8 H4.fcs · H4 DMSO.fcs · H4 origin firing DMSO EdU.fcs · mESC H4 DMSO.fcs |
| Sample Type | |
|---|---|
| · Biological replicate 1 | A17 DMSO.fcs · B24 DMSO.fcs · WT DMSO.fcs · U2OS A10 DMSO.fcs · U2OS A11 DMSO.fcs · U2OS WT DMSO.fcs · 0h CCNC D1.fcs · 0h CCNC G2.fcs · 0h CDK8 B9.fcs · 0h CDK8 H4.fcs · 0h WT.fcs |
| · Biological replicate 2 | A17 DMSO.fcs-(1) · B24 DMSO.fcs-(1) · WT DMSO.fcs-(1) · A10 DMSO.fcs · A11 DMSO.fcs · WT DMSO.fcs-(3) · B9 origin firing DMSO EdU.fcs · D1 Origin firing DMSO EdU.fcs · G2 origin firing DMSO EdU.fcs · H4 origin firing DMSO EdU.fcs · WT Origin firing DMSO EdU.fcs |
| · Biological replicate 3 | A17 DMSO.fcs-(2) · B24 DMSO.fcs-(2) · WT DMSO.fcs-(2) · A10 DMSO.fcs-(1) · A11 DMSO.fcs-(1) · WT DMSO.fcs-(4) · B9 DMSO.fcs · D1 DMSO.fcs · G2 DMSO.fcs · H4 DMSO.fcs · WT DMSO.fcs-(5) |
| · Biological replicate 4 | mESC B9 DMSO.fcs · mESC D1 DMSO.fcs · mESC G2 DMSO.fcs · mESC H4 DMSO.fcs · mESC WT DMSO.fcs |
