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Experiment Overview

Repository ID: FR-FCM-Z3FL Experiment name: AuxSen MIFlowCyt score: 86.00%
Primary researcher: Kenneth Berendzen PI/manager: Kenneth Berendzen Uploaded by: Kenneth Berendzen
Experiment dates: 2020-01-01 - 2021-02-04 Dataset uploaded: Feb 2021 Last updated: Feb 2021
Keywords: [plant cell suspension culture] [Aux/IAAs] [Arabidopsis] Manuscripts: Naturelogo
Organizations: Center for Plant Molecular Biology at the University of Tuebingen, FACS & Transformation, Tuebingen, BW (Germany)
Purpose: Monitor the FRET ratio of AuxSen reporter in response to Indole-3-acetic acid (IAA).
Conclusion: AuxSen responds to IAA in vivo
Comments: None
Funding: Not disclosed
Quality control: water, mNeonGreen and Aquamarine samples provide baselines and spillover boundaries. Alignment with Rainbow Calibration Particles P-RCP8-3.0 Kisker Biotech.


Experiment variables

Conditions
· reference Sample_189_H2O.fcs · Sample_190_AQUA.fcs · Sample_191_NEON.fcs
· control Sample_187_8p.fcs
· untreated Sample_193_AUX_0_0.fcs
· treated Sample_194_AUX_a_1.fcs · Sample_195_AUX_a_2.fcs · Sample_196_AUX_a_3.fcs · Sample_197_AUX_a_4.fcs · Sample_198_AUX_a_5.fcs · Sample_199_AUX_a_6.fcs · Sample_200_AUX_a_7.fcs · Sample_201_AUX_a_8.fcs · Sample_202_AUX_a_9.fcs · Sample_203_AUX_a_10.fcs · Sample_204_AUX_a_11.fcs · Sample_205_AUX_a_12.fcs · Sample_206_AUX_b_1.fcs · Sample_207_AUX_b_2.fcs · Sample_208_AUX_b_3.fcs · Sample_209_AUX_b_4.fcs · Sample_210_AUX_b_5.fcs · Sample_211_AUX_b_6.fcs · Sample_212_AUX_b_7.fcs · Sample_213_AUX_b_8.fcs · Sample_214_AUX_b_9.fcs · Sample_215_AUX_b_10.fcs · Sample_216_AUX_b_11.fcs · Sample_217_AUX_b_12.fcs · Sample_218_AUX_c_1.fcs · Sample_219_AUX_c_2.fcs · Sample_220_AUX_c_3.fcs · Sample_221_AUX_c_4.fcs · Sample_222_AUX_c_5.fcs · Sample_223_AUX_c_6.fcs · Sample_224_AUX_c_7.fcs · Sample_225_AUX_c_8.fcs · Sample_226_AUX_c_9.fcs · Sample_227_AUX_c_10.fcs · Sample_228_AUX_c_11.fcs · Sample_229_AUX_c_12.fcs

Doses
· 0 Sample_187_8p.fcs · Sample_189_H2O.fcs · Sample_190_AQUA.fcs · Sample_191_NEON.fcs · Sample_193_AUX_0_0.fcs
· 0.01 Sample_194_AUX_a_1.fcs · Sample_206_AUX_b_1.fcs · Sample_218_AUX_c_1.fcs
· 0.0025 Sample_195_AUX_a_2.fcs · Sample_207_AUX_b_2.fcs · Sample_219_AUX_c_2.fcs
· 0.000625 Sample_196_AUX_a_3.fcs · Sample_208_AUX_b_3.fcs · Sample_220_AUX_c_3.fcs
· 0.00015625 Sample_197_AUX_a_4.fcs · Sample_209_AUX_b_4.fcs · Sample_221_AUX_c_4.fcs
· 3.91E-05 Sample_198_AUX_a_5.fcs · Sample_210_AUX_b_5.fcs · Sample_222_AUX_c_5.fcs
· 9.77E-06 Sample_199_AUX_a_6.fcs · Sample_211_AUX_b_6.fcs · Sample_223_AUX_c_6.fcs
· 2.44E-06 Sample_200_AUX_a_7.fcs · Sample_212_AUX_b_7.fcs · Sample_224_AUX_c_7.fcs
· 6.10E-07 Sample_201_AUX_a_8.fcs · Sample_213_AUX_b_8.fcs · Sample_225_AUX_c_8.fcs
· 1.53E-07 Sample_202_AUX_a_9.fcs · Sample_214_AUX_b_9.fcs · Sample_226_AUX_c_9.fcs
· 3.81E-08 Sample_203_AUX_a_10.fcs · Sample_215_AUX_b_10.fcs · Sample_227_AUX_c_10.fcs
· 9.54E-09 Sample_204_AUX_a_11.fcs · Sample_216_AUX_b_11.fcs · Sample_228_AUX_c_11.fcs
· 2.38E-09 Sample_205_AUX_a_12.fcs · Sample_217_AUX_b_12.fcs · Sample_229_AUX_c_12.fcs

Timepoints
· 0 Sample_187_8p.fcs · Sample_189_H2O.fcs · Sample_190_AQUA.fcs · Sample_191_NEON.fcs
· 1 Sample_193_AUX_0_0.fcs · Sample_194_AUX_a_1.fcs · Sample_195_AUX_a_2.fcs · Sample_196_AUX_a_3.fcs · Sample_197_AUX_a_4.fcs · Sample_198_AUX_a_5.fcs · Sample_199_AUX_a_6.fcs · Sample_200_AUX_a_7.fcs · Sample_201_AUX_a_8.fcs · Sample_202_AUX_a_9.fcs · Sample_203_AUX_a_10.fcs · Sample_204_AUX_a_11.fcs · Sample_205_AUX_a_12.fcs · Sample_206_AUX_b_1.fcs · Sample_207_AUX_b_2.fcs · Sample_208_AUX_b_3.fcs · Sample_209_AUX_b_4.fcs · Sample_210_AUX_b_5.fcs · Sample_211_AUX_b_6.fcs · Sample_212_AUX_b_7.fcs · Sample_213_AUX_b_8.fcs · Sample_214_AUX_b_9.fcs · Sample_215_AUX_b_10.fcs · Sample_216_AUX_b_11.fcs · Sample_217_AUX_b_12.fcs · Sample_218_AUX_c_1.fcs · Sample_219_AUX_c_2.fcs · Sample_220_AUX_c_3.fcs · Sample_221_AUX_c_4.fcs · Sample_222_AUX_c_5.fcs · Sample_223_AUX_c_6.fcs · Sample_224_AUX_c_7.fcs · Sample_225_AUX_c_8.fcs · Sample_226_AUX_c_9.fcs · Sample_227_AUX_c_10.fcs · Sample_228_AUX_c_11.fcs · Sample_229_AUX_c_12.fcs

Individuals
· x Sample_189_H2O.fcs
· t Sample_187_8p.fcs
· y Sample_190_AQUA.fcs
· z Sample_191_NEON.fcs
· w Sample_193_AUX_0_0.fcs
· a Sample_194_AUX_a_1.fcs · Sample_195_AUX_a_2.fcs · Sample_196_AUX_a_3.fcs · Sample_197_AUX_a_4.fcs · Sample_198_AUX_a_5.fcs · Sample_199_AUX_a_6.fcs · Sample_200_AUX_a_7.fcs · Sample_201_AUX_a_8.fcs · Sample_202_AUX_a_9.fcs · Sample_203_AUX_a_10.fcs · Sample_204_AUX_a_11.fcs · Sample_205_AUX_a_12.fcs
· b Sample_206_AUX_b_1.fcs · Sample_207_AUX_b_2.fcs · Sample_208_AUX_b_3.fcs · Sample_209_AUX_b_4.fcs · Sample_210_AUX_b_5.fcs · Sample_211_AUX_b_6.fcs · Sample_212_AUX_b_7.fcs · Sample_213_AUX_b_8.fcs · Sample_214_AUX_b_9.fcs · Sample_215_AUX_b_10.fcs · Sample_216_AUX_b_11.fcs · Sample_217_AUX_b_12.fcs
· c Sample_218_AUX_c_1.fcs · Sample_219_AUX_c_2.fcs · Sample_220_AUX_c_3.fcs · Sample_221_AUX_c_4.fcs · Sample_222_AUX_c_5.fcs · Sample_223_AUX_c_6.fcs · Sample_224_AUX_c_7.fcs · Sample_225_AUX_c_8.fcs · Sample_226_AUX_c_9.fcs · Sample_227_AUX_c_10.fcs · Sample_228_AUX_c_11.fcs · Sample_229_AUX_c_12.fcs

Sample Type
· x Sample_189_H2O.fcs
· t Sample_187_8p.fcs
· y Sample_190_AQUA.fcs
· z Sample_191_NEON.fcs
· w Sample_193_AUX_0_0.fcs
· a Sample_194_AUX_a_1.fcs · Sample_195_AUX_a_2.fcs · Sample_196_AUX_a_3.fcs · Sample_197_AUX_a_4.fcs · Sample_198_AUX_a_5.fcs · Sample_199_AUX_a_6.fcs · Sample_200_AUX_a_7.fcs · Sample_201_AUX_a_8.fcs · Sample_202_AUX_a_9.fcs · Sample_203_AUX_a_10.fcs · Sample_204_AUX_a_11.fcs · Sample_205_AUX_a_12.fcs
· b Sample_206_AUX_b_1.fcs · Sample_207_AUX_b_2.fcs · Sample_208_AUX_b_3.fcs · Sample_209_AUX_b_4.fcs · Sample_210_AUX_b_5.fcs · Sample_211_AUX_b_6.fcs · Sample_212_AUX_b_7.fcs · Sample_213_AUX_b_8.fcs · Sample_214_AUX_b_9.fcs · Sample_215_AUX_b_10.fcs · Sample_216_AUX_b_11.fcs · Sample_217_AUX_b_12.fcs
· c Sample_218_AUX_c_1.fcs · Sample_219_AUX_c_2.fcs · Sample_220_AUX_c_3.fcs · Sample_221_AUX_c_4.fcs · Sample_222_AUX_c_5.fcs · Sample_223_AUX_c_6.fcs · Sample_224_AUX_c_7.fcs · Sample_225_AUX_c_8.fcs · Sample_226_AUX_c_9.fcs · Sample_227_AUX_c_10.fcs · Sample_228_AUX_c_11.fcs · Sample_229_AUX_c_12.fcs

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