Experiment Overview
| Repository ID: | FR-FCM-Z3HL | Experiment name: | Simonds et al 2021 -- Figure 1d-e (CyTOF mass cytometry of GL261 and SB28 mouse gliomas) | MIFlowCyt score: | 23.25% |
| Primary researcher: | Erin Simonds | PI/manager: | Erin Simonds | Uploaded by: | Erin Simonds |
| Experiment dates: | 2018-01-17 - 2018-01-17 | Dataset uploaded: | Feb 2021 | Last updated: | Jun 2021 |
| Keywords: | None | Manuscripts: | |||
| Organizations: |
UCSF, San Francisco, (USA)
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| Purpose: | Data corresponding to Figure 1d-e of http://doi.org/10.1136/jitc-2020-002181 Comparing abundance of immune cell populations in mouse GBM models SB28 and GL261 stained with the CyTOF mouse antibody panel (Suppl Table S4). Data were filtered on CD45-positive cells and processed using the PhenoGraph + FlowSOM analysis pipeline ("PhenoSOM") to segregate immune cell types into metaclusters and quantify their frequency across samples. The cluster IDs after Step 2 of the PhenoSOM pipeline are attached as CSV files (compressed as ZIP files). A key to link the FCS filenames to the cluster ID CSV files are also attached. | ||||
| Conclusion: | None | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Conditions | |
|---|---|
| · GL261 | FR-FCM-Z3HL - 01 - GL261 ic tumor_ViableSinglets.fcs |
| · SB28 | FR-FCM-Z3HL - 02 - SB28 ic tumor_ViableSinglets.fcs · FR-FCM-Z3HL - 03 - SB28 ic tumor_ViableSinglets.fcs · FR-FCM-Z3HL - 04 - SB28 ic tumor_ViableSinglets.fcs · FR-FCM-Z3HL - 05 - SB28 ic tumor_ViableSinglets.fcs |
| Sample Type | |
|---|---|
| · Dissociated intracerebral tumor | FR-FCM-Z3HL - 01 - GL261 ic tumor_ViableSinglets.fcs · FR-FCM-Z3HL - 02 - SB28 ic tumor_ViableSinglets.fcs · FR-FCM-Z3HL - 03 - SB28 ic tumor_ViableSinglets.fcs · FR-FCM-Z3HL - 04 - SB28 ic tumor_ViableSinglets.fcs · FR-FCM-Z3HL - 05 - SB28 ic tumor_ViableSinglets.fcs |
