Experiment Overview

Repository ID: FR-FCM-Z3J5 Experiment name: Fitness of GCR1 mutant strains of yeast in rich medium MIFlowCyt score: 21.75%
Primary researcher: Fabien Duveau PI/manager: Patricia Wittkopp Uploaded by: Fabien Duveau
Experiment dates: 2017-04-16 - 2017-04-17 Dataset uploaded: Mar 2021 Last updated: Mar 2021
Keywords: None Manuscripts:
Organizations: University of Michigan, Ann Arbor, MI (USA)
Purpose: The purpose of this assay is to measure the fitness of yeast strains with random mutations in the GCR1 coding sequence. These strains expressed a pTDH3-YFP reporter gene and were competed against a reference strain expressing a pTDH3-GFP reporter gene.
Conclusion: Mutations in GCR1 coding sequence that decrease expression of pTDH3-YFP (see experience FR-FCM-Z3J3) tend to be strongly deleterious.
Comments: None
Funding: Not disclosed
Quality control: None
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