Experiment Overview
Repository ID: | FR-FCM-Z3J6 | Experiment name: | COVID-19 immunophenotyping | MIFlowCyt score: | 27.75% |
Primary researcher: | guillaume carissimo | PI/manager: | guillaume carissimo | Uploaded by: | guillaume carissimo |
Experiment dates: | 2020-03-31 - 2021-05-12 | Dataset uploaded: | Mar 2021 | Last updated: | Mar 2021 |
Keywords: | [human whole blood immunophenotyping] [COVID-19] | Manuscripts: | [33067472] | ||
Organizations: |
Singapore Immunology Network, Singapore, (Singapore)
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Purpose: | Perform comprehensive immunophenotyping of SARS-CoV-2 symptomatic patients. | ||||
Conclusion: | Comprehensive flow cytometry of whole blood samples from 54 COVID-19 patients reveals a dramatic increase in the number of immature neutrophils. This increase strongly correlates with disease severity and is associated with elevated IL-6 and IP-10 levels, two key players in the cytokine storm. The most pronounced decrease in cell counts is observed for CD8 T-cells and VD2 γδ T-cells, which both exhibit increased differentiation and activation. ROC analysis reveals that the count ratio of immature neutrophils to VD2 (or CD8) T-cells predicts pneumonia onset (0.9071) as well as hypoxia onset (0.8908) with high sensitivity and specificity. | ||||
Comments: | During one day of acquisition the UV channel failed rapidly after QC. | ||||
Funding: | This work was supported by Singapore Immunology Network core research grant, the A*STAR COVID-19 Research funding (H/20/04/g1/006) provided to Singapore Immunology Network by the Biomedical Research Council (BMRC), A*STAR. Subject recruitment and sample collection were funded by the National Medical Research Council (NMRC) COVID-19 Research fund (COVID19RF-001). The SIgN flow cytometry and the Multiple analyte platforms were supported a grant from the National Research Foundation, Ïmmunomonitoring Service Platform ISP) (#NRF2017_SISFP09). | ||||
Quality control: | 8 peaks beads were ran before and after each experiment to control lasers. |