Experiment Overview

Repository ID: FR-FCM-Z3J6 Experiment name: COVID-19 immunophenotyping MIFlowCyt score: 27.75%
Primary researcher: guillaume carissimo PI/manager: guillaume carissimo Uploaded by: guillaume carissimo
Experiment dates: 2020-03-31 - 2021-05-12 Dataset uploaded: Mar 2021 Last updated: Mar 2021
Keywords: [human whole blood immunophenotyping] [COVID-19] Manuscripts: [33067472]
Organizations: Singapore Immunology Network, Singapore, (Singapore)
Purpose: Perform comprehensive immunophenotyping of SARS-CoV-2 symptomatic patients.
Conclusion: Comprehensive flow cytometry of whole blood samples from 54 COVID-19 patients reveals a dramatic increase in the number of immature neutrophils. This increase strongly correlates with disease severity and is associated with elevated IL-6 and IP-10 levels, two key players in the cytokine storm. The most pronounced decrease in cell counts is observed for CD8 T-cells and VD2 γδ T-cells, which both exhibit increased differentiation and activation. ROC analysis reveals that the count ratio of immature neutrophils to VD2 (or CD8) T-cells predicts pneumonia onset (0.9071) as well as hypoxia onset (0.8908) with high sensitivity and specificity.
Comments: During one day of acquisition the UV channel failed rapidly after QC.
Funding: This work was supported by Singapore Immunology Network core research grant, the A*STAR COVID-19 Research funding (H/20/04/g1/006) provided to Singapore Immunology Network by the Biomedical Research Council (BMRC), A*STAR. Subject recruitment and sample collection were funded by the National Medical Research Council (NMRC) COVID-19 Research fund (COVID19RF-001). The SIgN flow cytometry and the Multiple analyte platforms were supported a grant from the National Research Foundation, Ïmmunomonitoring Service Platform ISP) (#NRF2017_SISFP09).
Quality control: 8 peaks beads were ran before and after each experiment to control lasers.
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