Notice: Temporary Suspension of New Experiment Creation
We have temporarily disabled the creation of new experiments as we are continuously running out of space. This issue has been impacting both uploads and downloads from FlowRepository. By taking this step, we aim to make downloads of existing data more reliable.
We apologize for the inconvenience and appreciate your understanding as we work on upgrading our hardware and improving the overall solution.
Thank you for your patience.

Download-button_s

Experiment Overview

Repository ID: FR-FCM-Z3J6 Experiment name: COVID-19 immunophenotyping MIFlowCyt score: 27.75%
Primary researcher: guillaume carissimo PI/manager: guillaume carissimo Uploaded by: guillaume carissimo
Experiment dates: 2020-03-31 - 2021-05-12 Dataset uploaded: Mar 2021 Last updated: Mar 2021
Keywords: [human whole blood immunophenotyping] [COVID-19] Manuscripts: [33067472]
Organizations: Singapore Immunology Network, Singapore, (Singapore)
Purpose: Perform comprehensive immunophenotyping of SARS-CoV-2 symptomatic patients.
Conclusion: Comprehensive flow cytometry of whole blood samples from 54 COVID-19 patients reveals a dramatic increase in the number of immature neutrophils. This increase strongly correlates with disease severity and is associated with elevated IL-6 and IP-10 levels, two key players in the cytokine storm. The most pronounced decrease in cell counts is observed for CD8 T-cells and VD2 γδ T-cells, which both exhibit increased differentiation and activation. ROC analysis reveals that the count ratio of immature neutrophils to VD2 (or CD8) T-cells predicts pneumonia onset (0.9071) as well as hypoxia onset (0.8908) with high sensitivity and specificity.
Comments: During one day of acquisition the UV channel failed rapidly after QC.
Funding: This work was supported by Singapore Immunology Network core research grant, the A*STAR COVID-19 Research funding (H/20/04/g1/006) provided to Singapore Immunology Network by the Biomedical Research Council (BMRC), A*STAR. Subject recruitment and sample collection were funded by the National Medical Research Council (NMRC) COVID-19 Research fund (COVID19RF-001). The SIgN flow cytometry and the Multiple analyte platforms were supported a grant from the National Research Foundation, Ïmmunomonitoring Service Platform ISP) (#NRF2017_SISFP09).
Quality control: 8 peaks beads were ran before and after each experiment to control lasers.
Download FCS Files or login and see the dataset in your inbox for further annotation details.