Experiment Overview
| Repository ID: | FR-FCM-Z3MJ | Experiment name: | Sputum_analysis_LSRII_cytometer | MIFlowCyt score: | 95.17% |
| Primary researcher: | Jennifer Rebeles | PI/manager: | Jennifer Rebeles | Uploaded by: | Jennifer Rebeles |
| Experiment dates: | 2020-03-10 - 2020-03-11 | Dataset uploaded: | Apr 2021 | Last updated: | Jun 2021 |
| Keywords: | [flow cytometry] [Standardization] [Quality control] [sputum] [diagnostics] [lung disease] | Manuscripts: | |||
| Organizations: |
bioAffinity Technologies, bioAffinity Technologies, San Antonio, TX (United States)
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| Purpose: | To determine whether our method for sputum processing can be applied to different flow cytometric platforms. | ||||
| Conclusion: | Using our methods, we were able to successfully dissociate sputum into a single cell suspension into live, viable cells that were stained with blood and epithelial lineage markers and analyzed by three flow cytometric platforms. | ||||
| Comments: | Three-day sputum collection from persons aged 55-74 with at least a 30 pack-year smoking history at high risk of developing lung cancer. This experiment was used as a proof of concept of our protocol for a methods manuscript. No treatments or experimental conditions were applied to the samples. | ||||
| Funding: | n/a | ||||
| Quality control: | LSR II cytometer was checked prior to each analysis session with BD FACSDiva™ CS&T Research beads. The LSR II PMTs are adjusted and checked prior to each use using SPHERO™ Rainbow Calibration Particles. The presence of alveolar macrophages was verified to determine the sample derives from the lung. | ||||
