Experiment Overview
| Repository ID: | FR-FCM-Z3NE | Experiment name: | Impact of DRB on U2-OS cell cycle | MIFlowCyt score: | 94.92% |
| Primary researcher: | Rebecca Lloyd | PI/manager: | Steve Jackson | Uploaded by: | Rebecca Lloyd |
| Experiment dates: | 2021-01-05 - 2021-01-23 | Dataset uploaded: | Apr 2021 | Last updated: | Apr 2021 |
| Keywords: | [cell cycle] [DRB] | Manuscripts: | |||
| Organizations: |
University of Cambridge, Cambridge, (UK)
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| Purpose: | To establish whether DRB treatment impacts on the cell cycle and DNA replication in WT U2-OS cells, and the kinetics of these effects. | ||||
| Conclusion: | G1 accumulation and reduction in DNA replication following 100 uM DRB treatment for 6 hours. | ||||
| Comments: | Cells were treated for 0,1,2,4 or 6h with 100 uM DRB (transcription inhibitor). 30 min prior to fixation, cells were incubated with 10 uM EdU, a nucleotide analogue which is actively incorporated into actively replicating cells. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | non treated cells (0h DRB) established normal cell cycle distributions of WT U2-OS cells | ||||
Experiment variables
| Timepoints | |
|---|---|
| · 0h | 0h DRB.fcs |
| · 1h | 1h DRB.fcs |
| · 2h | 2h DRB.fcs |
| · 4h | 4h DRB.fcs |
| · 6h | 6h DRB.fcs |
| Doses | |
|---|---|
| · 100 uM | 1h DRB.fcs · 2h DRB.fcs · 4h DRB.fcs · 6h DRB.fcs |
| Individuals | |
|---|---|
| · WT U2-OS cells | 0h DRB.fcs · 1h DRB.fcs · 2h DRB.fcs · 4h DRB.fcs · 6h DRB.fcs |
