Experiment Overview
| Repository ID: | FR-FCM-Z3PG | Experiment name: | CRISPR library targeting lncRNA and protein coding genes along BLaER1 transdifferentiation (Replicate 1) | MIFlowCyt score: | 98.00% |
| Primary researcher: | Carme Arnan | PI/manager: | Carme Arnan | Uploaded by: | Carme Arnan |
| Experiment dates: | 2017-11-13 - 2017-11-13 | Dataset uploaded: | Apr 2021 | Last updated: | May 2022 |
| Keywords: | [macrophages] [CRISPR-Cas9 screening ] [BLaER cells] [pre-B cells] [transdifferentiation] | Manuscripts: | |||
| Organizations: |
Cytometry Unit Facility (CRG/UPF), Barcelona, Catalonia (SPAIN)
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| Purpose: | CRISPR library screening experiment in BLaER-Cas9 cells that were induced to transdifferentiate into macrophages. The purpose of the experiment is to discover novel players (lncRNAs and protein-coding genes) in the B-cell to macrophage transdifferentiation process. | ||||
| Conclusion: | There is a delayed population of cells that do not differentiate normally (this population is separated by cell sorting) in comparission to a control experiment with cells infected with an intergenic pgRNA | ||||
| Comments: | The experiment was done in 2 biological resplicates. | ||||
| Funding: | N/A | ||||
| Quality control: | BLaER-Cas9 cells infected with non-targeting negative control. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · T0 | 20171113_11 Lib T0.fcs · 20171113_8 Blaer T0.fcs · 20171113_9 S4 T0.fcs |
| · T3 | 20171113_12 Blaer 3D.fcs · 20171113_13 S4 3D.fcs · 20171113_15 Lib 3D.fcs · 20171113_20 Lib 3D.fcs |
| · T6 | 20171113_16 Blaer 6D.fcs · 20171113_17 S4 6D.fcs · 20171113_19 Lib 6D.fcs · 20171113_21 Lib 6D.fcs |
| Conditions | |
|---|---|
| · Controls | 20171113_12 Blaer 3D.fcs · 20171113_13 S4 3D.fcs · 20171113_16 Blaer 6D.fcs · 20171113_17 S4 6D.fcs · 20171113_8 Blaer T0.fcs · 20171113_9 S4 T0.fcs |
| · Samples | 20171113_11 Lib T0.fcs · 20171113_15 Lib 3D.fcs · 20171113_19 Lib 6D.fcs · 20171113_20 Lib 3D.fcs · 20171113_21 Lib 6D.fcs |
