Experiment Overview
| Repository ID: | FR-FCM-Z3PH | Experiment name: | CRISPR library targeting lncRNA and protein coding genes along BLaER1 transdifferentiation (Replicate 2) | MIFlowCyt score: | 98.00% |
| Primary researcher: | Carme Arnan | PI/manager: | Carme Arnan | Uploaded by: | Carme Arnan |
| Experiment dates: | 2018-03-02 - 2018-03-02 | Dataset uploaded: | Apr 2021 | Last updated: | May 2022 |
| Keywords: | [macrophages] [CRISPR-Cas9 screening ] [BLaER cells] [pre-B cells] [transdifferentiation] | Manuscripts: | |||
| Organizations: |
Cytometry Unit Facility (CRG/UPF), Barcelona, Catalonia (SPAIN)
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| Purpose: | CRISPR library screening experiment in BLaER-Cas9 cells that were induced to transdifferentiate into macrophages. The purpose of the experiment is to discover novel players (lncRNAs and protein-coding genes) in the B-cell to macrophage transdifferentiation process. | ||||
| Conclusion: | There is a delayed population of cells that do not differentiate normally (this population is separated by cell sorting) in comparission to a control experiment with cells infected with a intergenic pgRNA | ||||
| Comments: | The experiment was done in 2 biological resplicates (R1 and R2). | ||||
| Funding: | N/A | ||||
| Quality control: | BLaER Cas9 infected with non-targeting pgRNA negative control. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · T0 | 20180302_10 Lib T0.fcs · 20180302_8 Blaer T0.fcs · 20180302_9 S T0.fcs |
| · T3 | 20180302_11 Blaer T3.fcs · 20180302_12 S T3.fcs · 20180302_13 Lib T3.fcs · 20180302_17 ERC Lib T3 sort.fcs |
| · T6 | 20180302_14 Blaer 6D.fcs · 20180302_15 S T6.fcs · 20180302_16 ERC Lib T6.fcs · 20180302_18 ERC Lib T6 sort.fcs |
| Conditions | |
|---|---|
| · Controls | 20180302_11 Blaer T3.fcs · 20180302_12 S T3.fcs · 20180302_14 Blaer 6D.fcs · 20180302_15 S T6.fcs · 20180302_8 Blaer T0.fcs · 20180302_9 S T0.fcs |
| · Samples | 20180302_10 Lib T0.fcs · 20180302_13 Lib T3.fcs · 20180302_16 ERC Lib T6.fcs · 20180302_17 ERC Lib T3 sort.fcs · 20180302_18 ERC Lib T6 sort.fcs |
