Experiment Overview
| Repository ID: | FR-FCM-Z3PK | Experiment name: | Cell isolation by FACS of lncRNAs targeted with pgRNAs along BLaER1 transdifferentiation | MIFlowCyt score: | 98.00% |
| Primary researcher: | Carme Arnan | PI/manager: | Carme Arnan | Uploaded by: | Carme Arnan |
| Experiment dates: | 2019-03-04 - 2019-03-11 | Dataset uploaded: | Apr 2021 | Last updated: | May 2022 |
| Keywords: | [macrophages] [pre-B cells] [lncRNA validation] [delayed fraction] [BLaER-Cas9 transdiferentiation] | Manuscripts: | |||
| Organizations: |
Cytometry Unit Facility (CRG/UPF), Barcelona, Catalonia (SPAIN)
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| Purpose: | Validation of lncRNA targets from CRISPR screening in BLaER-Cas9 infected cells. | ||||
| Conclusion: | Enrichment of transdifferentiation delayed population for checking CRISPR knockout of lncRNAs validated targets. | ||||
| Comments: | N/A | ||||
| Funding: | N/A | ||||
| Quality control: | Non-targeted BLaER-Cas9 cells or cells targeted with intergenic pgRNAs. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · T0 | 20190304_5 T0 874 C.fcs · 20190304_7 T0 882 R1.fcs · 20190304_8 T0 882 R2.fcs · 20190311_5 T0 874 C.fcs · 20190311_7 T0 881 R1.fcs · 20190311_8 T0 881 R2.fcs |
| · T3 | 20190304_10 882 R2.fcs · 20190304_6 T3 874 C.fcs · 20190304_9 882 R1.fcs · 20190311_10 881 R2.fcs · 20190311_6 T3 874 C.fcs · 20190311_9 881 R1.fcs |
| Conditions | |
|---|---|
| · Negative control | 20190304_5 T0 874 C.fcs · 20190304_6 T3 874 C.fcs · 20190311_5 T0 874 C.fcs · 20190311_6 T3 874 C.fcs |
| · lncRNAs | 20190304_10 882 R2.fcs · 20190304_7 T0 882 R1.fcs · 20190304_8 T0 882 R2.fcs · 20190304_9 882 R1.fcs · 20190311_10 881 R2.fcs · 20190311_7 T0 881 R1.fcs · 20190311_8 T0 881 R2.fcs · 20190311_9 881 R1.fcs |
