Experiment Overview
| Repository ID: | FR-FCM-Z3PM | Experiment name: | Cell isolation by FACS of protein coding genes targeted with pgRNAs along BLaER1 transdifferentiation | MIFlowCyt score: | 98.00% |
| Primary researcher: | Carme Arnan | PI/manager: | Carme Arnan | Uploaded by: | Carme Arnan |
| Experiment dates: | 2019-05-03 - 2019-06-14 | Dataset uploaded: | Apr 2021 | Last updated: | May 2022 |
| Keywords: | [macrophages] [pre-B cells] [BLaER-Cas9 transdiferentiation] [FURIN] [NFE2] | Manuscripts: | |||
| Organizations: |
Cytometry Unit Facility (CRG/UPF), Barcelona, Catalonia (SPAIN)
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| Purpose: | Validate the effect of knocking out protein coding genes FURIN and NFE2 in the transdifferentiation of BLaER-Cas9 cells. | ||||
| Conclusion: | FURIN and NFE2 knockout could be further validated at RNA level (RT-qPCR) and protein level (western blot) from sorted cells. | ||||
| Comments: | None | ||||
| Funding: | N/A | ||||
| Quality control: | BLaER-Cas9 cells infected with intergenic pgRNAs. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · T0 | 20190503_4 Scramble T0.fcs · 20190503_6 Furin T0.fcs · 20190506_5 Scamble T0.fcs · 20190506_7 NFE2 T0.fcs · 20190614_10 NFE T0.fcs · 20190614_5 Scramble T0.fcs · 20190614_8 Furin T0.fcs |
| · T2 | 20190506_6 Scamble T2.fcs · 20190506_8 NFE2 T2.fcs · 20190614_6 Scramble T2.fcs · 20190614_11 NFE T2.fcs |
| · T3 | 20190503_5 Scramble T3.fcs · 20190503_7 Furin T3.fcs · 20190614_7 Scramble T3.fcs · 20190614_9 Furin T3.fcs |
| Conditions | |
|---|---|
| · Controls | 20190503_4 Scramble T0.fcs · 20190503_5 Scramble T3.fcs · 20190506_5 Scamble T0.fcs · 20190506_6 Scamble T2.fcs · 20190614_5 Scramble T0.fcs · 20190614_6 Scramble T2.fcs · 20190614_7 Scramble T3.fcs |
| · Samples | 20190503_6 Furin T0.fcs · 20190503_7 Furin T3.fcs · 20190506_7 NFE2 T0.fcs · 20190506_8 NFE2 T2.fcs · 20190614_10 NFE T0.fcs · 20190614_8 Furin T0.fcs · 20190614_9 Furin T3.fcs · 20190614_11 NFE T2.fcs |
| Sample Type | |
|---|---|
| · R1 | 20190503_4 Scramble T0.fcs · 20190503_5 Scramble T3.fcs · 20190503_6 Furin T0.fcs · 20190503_7 Furin T3.fcs · 20190506_6 Scamble T2.fcs · 20190506_7 NFE2 T0.fcs · 20190506_8 NFE2 T2.fcs |
| · R2 | 20190506_5 Scamble T0.fcs · 20190614_10 NFE T0.fcs · 20190614_5 Scramble T0.fcs · 20190614_6 Scramble T2.fcs · 20190614_7 Scramble T3.fcs · 20190614_8 Furin T0.fcs · 20190614_9 Furin T3.fcs · 20190614_11 NFE T2.fcs |
