Experiment Overview
| Repository ID: | FR-FCM-Z3PW | Experiment name: | Validation of candidate hits along BLaER1 transdifferentiation (Replicate 1) | MIFlowCyt score: | 98.00% |
| Primary researcher: | Carme Arnan | PI/manager: | Carme Arnan | Uploaded by: | Carme Arnan |
| Experiment dates: | 2018-10-05 - 2018-10-08 | Dataset uploaded: | Apr 2021 | Last updated: | May 2022 |
| Keywords: | [macrophages] [transdifferentiation] [Target validation] [pc-genes] [lncRNAs] [BLaER (pre-B cells)] | Manuscripts: | |||
| Organizations: |
Cytometry Unit Facility (CRG/UPF), Barcelona, Catalonia (SPAIN)
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| Purpose: | Validation of hits from CRISPR-Cas9 screening library in BLaER cells (replicate 1). | ||||
| Conclusion: | 5 protein coding genes(CEACAM, FURIN, KLF4, NFE2 and TREML2) and 2 lncRNAs (LINC02432 and MIR3945HG) were individually validated. | ||||
| Comments: | None | ||||
| Funding: | N/A | ||||
| Quality control: | BLaER Cas9 infected with Intergenic pgRNA. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · T0 | 20181005_10 Furin 870 t0.fcs · 20181005_11 KLF4 873 t0.fcs · 20181005_12 Unk 874 t0.fcs · 20181005_13 Spi1 875 t0.fcs · 20181005_14 CEACAM 876 t0.fcs · 20181005_15 NFE 877 t0.fcs · 20181005_16 TREML 878 t0.fcs · 20181005_19 881 t0.fcs · 20181005_20 882 t0.fcs · 20181005_8 Blaer Cas9 t0.fcs · 20181005_9 rat cebpa 869 t0.fcs · 20181008_10 Furin 870 t0.fcs · 20181008_11 KLF4 873 t0.fcs · 20181008_12 Unk 874 t0.fcs · 20181008_13 Spi1 875 t0.fcs · 20181008_14 CEACAM 876 t0.fcs · 20181008_15 NFE 877 t0.fcs · 20181008_16 TREML 878 t0.fcs · 20181008_19 881 t0.fcs · 20181008_20 882 t0.fcs · 20181008_8 Blaer Cas9 t0.fcs · 20181008_9 rat cebpa 869 t0.fcs |
| · T3 | 20181005_22 Blaer Cas9 t3.fcs · 20181005_23 rat cebpa 869 t3.fcs · 20181005_24 Furin 870 t3.fcs · 20181005_25 KLF4873 t3.fcs · 20181005_26 Unk 874 t3.fcs · 20181005_27 Spi1 875 t3.fcs · 20181005_28 CEACAM 876 t3.fcs · 20181005_29 NFE 877 t3.fcs · 20181005_30 TREML 878 t3.fcs · 20181005_33 881 t3.fcs · 20181005_34 882 t3.fcs |
| · T6 | 20181008_22 Blaer Cas9 t6.fcs · 20181008_23 rat cebpa 869 t6.fcs · 20181008_24 Furin 870 t6.fcs · 20181008_25 KLF4873 t6.fcs · 20181008_26 Unk 874 t6.fcs · 20181008_27 Spi1 875 t6.fcs · 20181008_28 CEACAM 876 t6.fcs · 20181008_29 NFE 877 t6.fcs · 20181008_30 TREML 878 t6.fcs · 20181008_33 881 t6.fcs · 20181008_34 882 t6.fcs |
| Conditions | |
|---|---|
| · Protein coding genes | 20181005_10 Furin 870 t0.fcs · 20181005_11 KLF4 873 t0.fcs · 20181005_14 CEACAM 876 t0.fcs · 20181005_15 NFE 877 t0.fcs · 20181005_16 TREML 878 t0.fcs · 20181005_24 Furin 870 t3.fcs · 20181005_25 KLF4873 t3.fcs · 20181005_28 CEACAM 876 t3.fcs · 20181005_29 NFE 877 t3.fcs · 20181005_30 TREML 878 t3.fcs · 20181008_10 Furin 870 t0.fcs · 20181008_11 KLF4 873 t0.fcs · 20181008_14 CEACAM 876 t0.fcs · 20181008_15 NFE 877 t0.fcs · 20181008_16 TREML 878 t0.fcs · 20181008_24 Furin 870 t6.fcs · 20181008_25 KLF4873 t6.fcs · 20181008_28 CEACAM 876 t6.fcs · 20181008_29 NFE 877 t6.fcs · 20181008_30 TREML 878 t6.fcs |
| · lncRNAs | 20181005_19 881 t0.fcs · 20181005_20 882 t0.fcs · 20181005_33 881 t3.fcs · 20181005_34 882 t3.fcs · 20181008_19 881 t0.fcs · 20181008_20 882 t0.fcs · 20181008_33 881 t6.fcs · 20181008_34 882 t6.fcs |
| · positive controls | 20181005_13 Spi1 875 t0.fcs · 20181005_23 rat cebpa 869 t3.fcs · 20181005_27 Spi1 875 t3.fcs · 20181005_9 rat cebpa 869 t0.fcs · 20181008_13 Spi1 875 t0.fcs · 20181008_23 rat cebpa 869 t6.fcs · 20181008_27 Spi1 875 t6.fcs · 20181008_9 rat cebpa 869 t0.fcs |
| · Negative controls | 20181005_12 Unk 874 t0.fcs · 20181005_22 Blaer Cas9 t3.fcs · 20181005_26 Unk 874 t3.fcs · 20181005_8 Blaer Cas9 t0.fcs · 20181008_12 Unk 874 t0.fcs · 20181008_22 Blaer Cas9 t6.fcs · 20181008_26 Unk 874 t6.fcs · 20181008_8 Blaer Cas9 t0.fcs |
