Experiment Overview
| Repository ID: | FR-FCM-Z3RU | Experiment name: | Quantification of AdVP transduction levels in hMSCs | MIFlowCyt score: | 77.50% |
| Primary researcher: | Qian Wang | PI/manager: | Manuel Goncalves | Uploaded by: | Qian Wang |
| Experiment dates: | 2020-07-14 - 2020-07-21 | Dataset uploaded: | May 2021 | Last updated: | Sep 2021 |
| Keywords: | [hMSCs] [AdVP.mCherry] [transduction efficiency] | Manuscripts: | [34669958] [PMC8599732] | ||
| Organizations: |
Leiden University Medical Center, Cell and Chemical Biology, Leiden, South Holland (Netherlands)
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| Purpose: | Determining AdVP transduction efficiency in hMSCs. | ||||
| Conclusion: | Transduction efficiencies, as determined by flow cytometry, varied from a minimum of 92.4±2.2% to a maximum of 99.23±0.8%. | ||||
| Comments: | The hMSCs were exposed to the indicated amounts of AdVP.mCherry. At 3 days post-transduction, the frequencies of transduced cells and the protein expression levels per transduced cell, were assessed by mCherry-directed flow cytometry. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Untransduced mock cells served to establish fluorescence background thresholds. At least 10,000 viable single cells were acquired per sample | ||||
