Experiment Overview
| Repository ID: | FR-FCM-Z3TM | Experiment name: | Ratios RI, FP & EC | MIFlowCyt score: | 19.50% |
| Primary researcher: | Charlotte van de Velde | PI/manager: | Karoline Faust | Uploaded by: | Charlotte van de Velde |
| Experiment dates: | 2020-09-04 - | Dataset uploaded: | May 2021 | Last updated: | May 2021 |
| Keywords: | None | Manuscripts: | |||
| Organizations: |
KU Leuven, Leuven, Vlaams Brabant (Belgium)
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| Purpose: | Quantification of mixed ratios of different gut-bacterial pairs: Roseburia intestinalis + Faecalibacterium prausnitzii (RI-FP), Roseburia intestinalis + Escherichia coli with mCherry expressed (RI-EC), Faecalibacterium prausnitzii + Escherichia coli with mCherry expressed (FP-EC) | ||||
| Conclusion: | None | ||||
| Comments: | Initial monocultures of the three strains were measured and all monocultures were diluted in PBS to the same concentration. Subsequently, the monocultures were mixed in different ratios (5%-95%, as described in the sample names). In addition, monoculture ratios were also made in PBS (5%-95%) to approximate cell counts in the mixtures. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
