Experiment Overview
| Repository ID: | FR-FCM-Z3V2 | Experiment name: | PEX overexpression H2DCFDA | MIFlowCyt score: | 29.25% |
| Primary researcher: | Joe Delaney | PI/manager: | Joe Delaney | Uploaded by: | Joe Delaney |
| Experiment dates: | 2018-08-20 - 2018-08-31 | Dataset uploaded: | Jun 2021 | Last updated: | Jun 2021 |
| Keywords: | [Cancer] [reactive oxygen species] [chemotherapy] [chemoresistance] | Manuscripts: | |||
| Organizations: |
Medical University of South Carolina, Biochemistry and Molecular Biology, Charleston, SC (USA)
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| Purpose: | Assessment of reactive oxygen species (ROS) using H2DCFDA stained cells in the presence or absence of cisplatin-mediated stress. PEX5 and PEX19 were overexpressed and compared to control vectors. | ||||
| Conclusion: | PEX5 and PEX19 each reduced ROS steady state levels after cisplatin treatment | ||||
| Comments: | None | ||||
| Funding: | National Institutes of Health, National Cancer Institute, CA207729 | ||||
| Quality control: | Unstained cells were used to determine basal green-channel fluorescence for all constructs and cell lines. Propidium Iodide was used to gate out dead cells, as was size selection of small apoptotic blebs. Standard methods were used to gate out doublets. | ||||
