Experiment Overview
| Repository ID: | FR-FCM-Z3WV | Experiment name: | Secondary screen of pTDH3-YFP expression changes in yeast EMS mutants | MIFlowCyt score: | 23.25% |
| Primary researcher: | Fabien Duveau | PI/manager: | Patricia Wittkopp | Uploaded by: | Fabien Duveau |
| Experiment dates: | 2015-08-17 - | Dataset uploaded: | Mar 2021 | Last updated: | Mar 2021 |
| Keywords: | None | Manuscripts: | |||
| Organizations: |
University of Michigan, Ann Arbor, MI (USA)
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| Purpose: | The purpose of the experiment was to identify EMS mutants strains with altered expression of the pTDH3-YFP reporter gene (YFP expressed under control of the TDH3 promoter in yeast cells). 6 replicate populations of 315 mutant strains were grown separately in YPD medium before measuring fluorescence of >1000 cells for each sample on a BD C6 Accuri instrument. | ||||
| Conclusion: | Quantitative analysis of flow cytometry data showed a significant change of expression in 174 of the 315 mutants. 44 of these mutants were then selected for mapping mutations altering pTDH3-YFP expression in the yeast genome. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | The experiment includes several replicate populations of a strain that does not contain the pTDH3-YFP reporter gene and that is used to estimate the level of autofluorescence. 120 samples correspond to replicate populations of the wild-type progenitor strain used as a reference to quantify expression changes in each mutant. An additional 24 wild-type strains expressing pTDH3-YFP were arrayed at predetermined positions in each 96-well plates to correct for technical variation between plates or flow cytometry runs. | ||||
